{"id":67372,"date":"2026-06-12T07:25:23","date_gmt":"2026-06-12T05:25:23","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/06\/12\/runx2-promotes-chromatin-accessibility-and-wnt-signaling-in-inflamed-intestinal-epithelial-cells-rodolfo-i-cabrera-silva\/"},"modified":"2026-06-12T07:25:23","modified_gmt":"2026-06-12T05:25:23","slug":"runx2-promotes-chromatin-accessibility-and-wnt-signaling-in-inflamed-intestinal-epithelial-cells-rodolfo-i-cabrera-silva","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/06\/12\/runx2-promotes-chromatin-accessibility-and-wnt-signaling-in-inflamed-intestinal-epithelial-cells-rodolfo-i-cabrera-silva\/","title":{"rendered":"RUNX2 promotes chromatin accessibility and WNT signaling in inflamed intestinal epithelial cells. Rodolfo I Cabrera-Silva"},"content":{"rendered":"<div>\n<p><b>Mucosal Immunol<\/b>. 2026 Jun 11:100361. doi: 10.1016\/j.mucimm.2026.100361. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Ulcerative colitis (UC) is characterized by chronic mucosal inflammation, recurrent epithelial injury, and impaired colonic mucosal wound healing. While WNT\/\u03b2-catenin dysregulation has been reported in UC, the mechanisms of such abnormalities remain unclear. To investigate epithelial intrinsic alterations associated with UC, we performed single-nucleus RNA-seq (snRNA-seq) and ATAC-seq (snATAC-seq) multiomics on human primary colonic epithelial cells (colonoids) from healthy donors and patients with inactive or active UC. Colonoids were cultured in a 3D matrix recapitulating crypt base cells or grown as 2D monolayers in differentiation medium to recapitulate luminal epithelial cells. Colonoids from active UC had a unique cell population with elevated CTNNB1 and reduced APC expression. Chromatin profiling identified enrichment of RUNX2 motifs in this UC-associated cell population. Active UC colonoids exhibited reduced OLFM4 expression in 3D and the differentiation marker VIL1 in 2D, suggesting impaired epithelial stem-cell maintenance and maturation. RUNX2 inhibition using CADD522 reduced \u03b2-catenin levels in 3D colonoids and restored VIL1 expression and junctional \u03b2-catenin localization in 2D cultures. These findings reveal an intrinsic defect in epithelial renewal in UC, driven in part by RUNX2-dependent WNT dysregulation. Our study identifies RUNX2 as a transcriptional regulator of epithelial stem cell function and WNT signaling in the inflamed human colon.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/42276192\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=101299742&amp;ff=20260612012522&amp;v=2.20.0\">42276192<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1016\/j.mucimm.2026.100361\">10.1016\/j.mucimm.2026.100361<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Mucosal Immunol. 2026 Jun 11:100361. doi: 10.1016\/j.mucimm.2026.100361. Online ahead of print. ABSTRACT Ulcerative colitis (UC) is characterized by chronic mucosal inflammation, recurrent epithelial injury, and impaired colonic mucosal wound healing. While WNT\/\u03b2-catenin dysregulation has been reported in UC, the mechanisms of such abnormalities remain unclear. To investigate epithelial intrinsic alterations associated with UC, we performed &#8230; <a title=\"RUNX2 promotes chromatin accessibility and WNT signaling in inflamed intestinal epithelial cells. Rodolfo I Cabrera-Silva\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/06\/12\/runx2-promotes-chromatin-accessibility-and-wnt-signaling-in-inflamed-intestinal-epithelial-cells-rodolfo-i-cabrera-silva\/\" aria-label=\"Read more about RUNX2 promotes chromatin accessibility and WNT signaling in inflamed intestinal epithelial cells. Rodolfo I Cabrera-Silva\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[57,42],"tags":[],"class_list":["post-67372","post","type-post","status-publish","format-standard","hentry","category-mucosal-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/67372","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=67372"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/67372\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=67372"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=67372"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=67372"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}