{"id":69050,"date":"2026-07-02T06:50:15","date_gmt":"2026-07-02T04:50:15","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/07\/02\/4-1bbl-on-monocyte-lineage-cells-rather-than-on-classical-dendritic-cells-drives-cd8-t-cell-accumulation-in-the-respiratory-tract-and-protects-from-severe-respiratory-influenza-infection-karen-k-m-y\/"},"modified":"2026-07-02T06:50:15","modified_gmt":"2026-07-02T04:50:15","slug":"4-1bbl-on-monocyte-lineage-cells-rather-than-on-classical-dendritic-cells-drives-cd8-t-cell-accumulation-in-the-respiratory-tract-and-protects-from-severe-respiratory-influenza-infection-karen-k-m-y","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/07\/02\/4-1bbl-on-monocyte-lineage-cells-rather-than-on-classical-dendritic-cells-drives-cd8-t-cell-accumulation-in-the-respiratory-tract-and-protects-from-severe-respiratory-influenza-infection-karen-k-m-y\/","title":{"rendered":"4-1BBL on monocyte lineage cells rather than on classical dendritic cells drives CD8+ T cell accumulation in the respiratory tract and protects from severe respiratory influenza infection. Karen K M Yeung"},"content":{"rendered":"<div>\n<p><b>Mucosal Immunol<\/b>. 2026 Jul 1:100374. doi: 10.1016\/j.mucimm.2026.100374. Online ahead of print.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>Seasonal epidemics and the persistent threat of a pandemic provide a strong impetus to understand mechanisms of protection against influenza infection. T cell intrinsic signaling through the TNFR superfamily member 4-1BB is critical for the accumulation of antigen-specific CD8<sup>+<\/sup> effector and memory T cells in the lung and protection from influenza A virus (IAV). However, the APCs that provide 4-1BB ligand (4-1BBL) have not been definitively characterized. Here, using single-cell RNA sequencing and multiparameter flow cytometry, we define murine monocyte lineage cell (MC) and classical dendritic cell (cDC) populations in the lung during acute IAV infection and show that 4-1BBL is more highly expressed on CD64<sup>+<\/sup>MAR-I<sup>+<\/sup>CD26<sup>&#8211;<\/sup> inflammatory MCs than on MHCII<sup>hi<\/sup>CD11c<sup>+<\/sup>CD26<sup>+<\/sup> cDCs following IAV infection. Mixed bone marrow chimeras, in which 4-1BBL is only absent on Ccr2-dependent cells, and Cre-driven deletion using Ccr2- or Zbtb46-cre demonstrate that 4-1BBL on MCs rather than cDCs is important for the accumulation of nucleoprotein (NP)-specific CD8<sup>+<\/sup> effector and tissue-resident memory T cells. Importantly, 4-1BBL on Ccr2-dependent cells is critical for mouse survival following severe IAV infection. These findings reveal a division of labor between cDC and MCs, with infMCs uniquely providing 4-1BBL to increase CD8<sup>+<\/sup> T cell accumulation in the lung and protect against severe IAV infection.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/42386082\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=101299742&amp;ff=20260702005014&amp;v=2.20.0\">42386082<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1016\/j.mucimm.2026.100374\">10.1016\/j.mucimm.2026.100374<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Mucosal Immunol. 2026 Jul 1:100374. doi: 10.1016\/j.mucimm.2026.100374. Online ahead of print. ABSTRACT Seasonal epidemics and the persistent threat of a pandemic provide a strong impetus to understand mechanisms of protection against influenza infection. T cell intrinsic signaling through the TNFR superfamily member 4-1BB is critical for the accumulation of antigen-specific CD8+ effector and memory T &#8230; <a title=\"4-1BBL on monocyte lineage cells rather than on classical dendritic cells drives CD8+ T cell accumulation in the respiratory tract and protects from severe respiratory influenza infection. Karen K M Yeung\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/07\/02\/4-1bbl-on-monocyte-lineage-cells-rather-than-on-classical-dendritic-cells-drives-cd8-t-cell-accumulation-in-the-respiratory-tract-and-protects-from-severe-respiratory-influenza-infection-karen-k-m-y\/\" aria-label=\"Read more about 4-1BBL on monocyte lineage cells rather than on classical dendritic cells drives CD8+ T cell accumulation in the respiratory tract and protects from severe respiratory influenza infection. Karen K M Yeung\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[57,42],"tags":[],"class_list":["post-69050","post","type-post","status-publish","format-standard","hentry","category-mucosal-immunology","category-publicaciones"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/69050","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=69050"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/69050\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=69050"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=69050"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=69050"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}