{"id":71992,"date":"2026-07-31T13:17:06","date_gmt":"2026-07-31T11:17:06","guid":{"rendered":"https:\/\/inmuno.es\/index.php\/2026\/07\/31\/rag1-based-time-stamp-tracing-of-t-cell-developmental-origins\/"},"modified":"2026-07-31T13:17:06","modified_gmt":"2026-07-31T11:17:06","slug":"rag1-based-time-stamp-tracing-of-t-cell-developmental-origins","status":"publish","type":"post","link":"https:\/\/inmuno.es\/index.php\/2026\/07\/31\/rag1-based-time-stamp-tracing-of-t-cell-developmental-origins\/","title":{"rendered":"Rag1-based time-stamp tracing of T-cell developmental origins"},"content":{"rendered":"<div>\n<p><b>J Immunol<\/b>. 2026 Jul 10;215(7):vkag154. doi: 10.1093\/jimmun\/vkag154.<\/p>\n<p><b>ABSTRACT<\/b><\/p>\n<p>The postnatal T\u2011cell pool comprises cells at diverse developmental stages, complicating efforts to resolve T\u2011cell differentiation, maturation, and long\u2011term adaptation. To address this challenge, we developed Rag1\u2011mediated time-stamp tracing, a genetic strategy that enables time\u2011resolved tracking of T\u2011cell developmental origins. We validate the specificity and temporal resolution of this approach across multiple analytical platforms, including flow cytometry, imaging, and single\u2011cell multiomics. Applying this strategy to thymic regulatory T (Treg) cell development, we show that time-stamp tracing resolves distinct phases of Treg cell induction, maturation, and long\u2011term adaptation within heterogeneous populations. This analysis identifies precursor populations for thymic Treg cell induction, uncovers a maturation program in nascent Treg cells marked by CD73 upregulation and progressive DNA hypomethylation at Treg cell-specific loci, and distinguishes long\u2011lived mature Treg cells characterized by activation and clonal expansion. Together, these findings establish Rag1\u2011based time-stamp tracing as a versatile and unbiased approach for resolving T\u2011cell developmental origins across immunological contexts.<\/p>\n<p>PMID:<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/42533592\/?utm_source=SimplePie&amp;utm_medium=rss&amp;utm_content=2985117R&amp;ff=20260731071705&amp;v=2.20.0.post5+40e1b98\">42533592<\/a> | DOI:<a href=\"https:\/\/doi.org\/10.1093\/jimmun\/vkag154\">10.1093\/jimmun\/vkag154<\/a><\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>J Immunol. 2026 Jul 10;215(7):vkag154. doi: 10.1093\/jimmun\/vkag154. ABSTRACT The postnatal T\u2011cell pool comprises cells at diverse developmental stages, complicating efforts to resolve T\u2011cell differentiation, maturation, and long\u2011term adaptation. To address this challenge, we developed Rag1\u2011mediated time-stamp tracing, a genetic strategy that enables time\u2011resolved tracking of T\u2011cell developmental origins. We validate the specificity and temporal resolution &#8230; <a title=\"Rag1-based time-stamp tracing of T-cell developmental origins\" class=\"read-more\" href=\"https:\/\/inmuno.es\/index.php\/2026\/07\/31\/rag1-based-time-stamp-tracing-of-t-cell-developmental-origins\/\" aria-label=\"Read more about Rag1-based time-stamp tracing of T-cell developmental origins\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42,71],"tags":[],"class_list":["post-71992","post","type-post","status-publish","format-standard","hentry","category-publicaciones","category-the-journal-of-immunology"],"_links":{"self":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/71992","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/comments?post=71992"}],"version-history":[{"count":0,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/posts\/71992\/revisions"}],"wp:attachment":[{"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/media?parent=71992"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/categories?post=71992"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/inmuno.es\/index.php\/wp-json\/wp\/v2\/tags?post=71992"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}