J Immunol. 2026 Oct 1;215(10):vkag274. doi: 10.1093/jimmun/vkag274.
ABSTRACT
A new human xeno-free T cell expansion strategy enabled T cell receptor α/β (TCRα/β)-driven autologous reactivity and continuous long-term IL-2-dependent expansion of an unconventional professional T cell antigen-presenting cell (T-APC) subset. Autologous T-APC cultures were obtained from small peripheral blood mononuclear cell samples that yielded over 1020-fold expansions by an approach that did not require cell purification or genetic manipulation. Primary T-APCs constitutively expressed major histocompatibility complex class II glycoproteins, costimulatory B7 molecules, and FOXP3 in long-term culture. Autologous expansion yielded both CD4+ and CD8+ T-APC subsets that expressed signature activation markers and rapid cell division over 1 to 2 mo of culture in the absence of exogenous mitogenic or antigenic stimuli. Autologous IL-2-driven expansion of CD4+ T-APCs required TCR engagement because continuous T cell proliferation was blocked by neutralizing monoclonal antibodies against CD4, and constitutive expression of CD40L was inhibited by neutralizing major histocompatibility complex class II monoclonal antibodies. Long-term expansion required continuous TGF-β exposure, which increased HLA-DR and CD25 expression with consequent enhancement of antigen presentation, autologous TCR recognition, and IL-2 responsiveness. Low IL-2/TGF-β concentrations favored CD25high CD69low PD1low T-APC subsets, whereas high IL-2/TGF-β concentrations favored CD25low CD69high PD1high T-APC subsets. T-APC presentation of agonistic self-antigens induced extensive blastogenesis of polyclonal syngeneic responder T cells, some of which differentiated de novo into exponentially expanding T-APC lines. Overall, this study reveals professional T-APC subsets that exhibit exponential autologous expansion in vitro via autologous self-presentation and agonistic TCR self-recognition. These T-APCs may regulate homeostatic self-reactivity of the human TCRα/β repertoire and may mediate, in part, autologous mixed lymphocyte reactions.
PMID:42906129 | DOI:10.1093/jimmun/vkag274