ADAM17-mediated CD16 shedding drives monocyte subset misclassification in ex vivo immunological assays
Human monocytes are classified as classical (CD14++CD16−), intermediate (CD14++CD16+), and non-classical (CD14+CD16++) subsets, but CD16 stability during ex vivo assays remains uncertain. Here, we examined whether standard PBMC handling alters CD16-bas…