Fast Generation of F(Ab’)2 Fragments From Human IgG Using Fc‐Fused IgG‐Degrading Enzyme. [[{“value”:”Emna Hannachi, Robin V. Lacombe, Amélia Trecco, Victoria Daventure, Sandrine Delignat, Jordan D. Dimitrov, Claire Deligne, Sébastien Lacroix‐Desmazes”}]]

Removal of Fc-fragments from human IgG or Fc-fused proteins. (1) IgG or Fc-fusion proteins are incubated with IdeS-Fc; (2) Fc fragments are retained using protein G agarose beads; and (3) the F(ab’)₂ fragments or Fc-free proteins are collected by centrifugation in the original buffer without IdeS contamination.