- Towards the Next Generation of Data‐Driven Therapeutics Using Spatially Resolved Single‐Cell Technologies and Generative AI. [[{“value”:”Avital Rodov,
Hosna Baniadam,
Robert Zeiser,
Ido Amit,
Nir Yosef,
Tobias Wertheimer,
Florian Ingelfinger”}]]by inmunoadminThis review explores the technical advances in single-cell multi-omics technologies alongside the parallel development of generative AI tools to uncover biological insights. By integrating these innovative approaches, we highlight the potential to identify diagnostic, prognostic and outcome biomarkers, enhance patient stratification systems and discuss future prospects for advancing precision medicine and personalised therapeutic strategies. ABSTRACT … Read more
- Cover Story: Eur. J. Immunol. 2’25.by inmunoadminOur cover features images related to flow cytometry techniques widely used for analysis of function and phenotypes of major human and murine immune cell subsets, superimposed on a multidimensional immune cell population scatter plot. These images are taken from the third edition of EJI’s Flow Cytometry Guidelines by Cossarizza et al., a comprehensive resource prepared … Read more
- Issue Information: Eur. J. Immunol. 2’25.by inmunoadminEuropean Journal of Immunology, Volume 55, Issue 2, February 2025.
- Striking the Right Chord at EJI: Introducing Editor‐in‐Chief Matteo Iannacone and the Seamless Transfer Policy. [[{“value”:”Matteo Iannacone,
Nadja Bakocevic”}]]by inmunoadminEuropean Journal of Immunology, Volume 55, Issue 1, January 2025.
- The Anti‐Human P2X7 Monoclonal Antibody (Clone L4) Can Mediate Complement‐Dependent Cytotoxicity of Human Leukocytes. [[{“value”:”Amal Elhage,
Debbie Watson,
Ronald Sluyter”}]]by inmunoadminThe anti-P2X7 monoclonal antibody (clone L4) mediates complement-dependent cytotoxicity of human leukocytes. This process preferentially targets leukocytes with high cell surface P2X7, such as monocytes and dendritic cells, presenting a new strategy to reduce P2X7 immune functions. ABSTRACT P2X7 is an extracellular adenosine 5′-triphosphate (ATP)-gated cation channel that plays various roles in inflammation and immunity. … Read more
- The VEGF‐Mediated Cytoprotective Ability of MIF‐Licensed Mesenchymal Stromal Cells in House Dust Mite‐Induced Epithelial Damage. [[{“value”:”Hazel Dunbar,
Ian J. Hawthorne,
Courteney Tunstead,
Molly Dunlop,
Evelina Volkova,
Daniel J. Weiss,
Claudia C. dos Santos,
Michelle E. Armstrong,
Seamas C. Donnelly,
Karen English”}]]by inmunoadminLicensing of MSCs with MIF requires CXCR4 receptor binding. Enhanced VEGF production by MIF-licensed MSCs promotes wound healing in vitro and can be blocked using the VEGFR2 inhibitor SU-5416. MIF-licensed MSC conditioned medium significantly reduced goblet cell hyperplasia in house dust mite-driven allergic airway inflammation in vivo. ABSTRACT Enhancing mesenchymal stromal cell (MSC) therapeutic efficacy … Read more
- Issue Information: Eur. J. Immunol. 1’25.by inmunoadminEuropean Journal of Immunology, Volume 55, Issue 1, January 2025.
- Direct Inhibitory Effect of HTLV‐1‐Infected T Cells on the Production of Anti‐Ro/SS‐A Antibody by B Cells from Patients with Sjögren’s Syndrome. [[{“value”:”Kinya Nagata,
Masako Tsukamoto,
Yosuke Nagasawa,
Noboru Kitamura,
Hideki Nakamura”}]]by inmunoadminHTLV-1-infected T-cell lines suppress IgG and autoantibody production from B cells of healthy donors and patients with Sjögren’s syndrome through cell-to-cell contact and some humoral factors. ABSTRACT The reasons for the low frequency of anti-Ro/SS-A antibody in patients with HTLV-1-associated myelopathy complicated with Sjögren’s syndrome (SS) are unclear. In this study, we investigated whether HTLV-1-infected … Read more
- Cover Story: Eur. J. Immunol. 1’25.by inmunoadminOur cover features images related to flow cytometry techniques widely used for analysis of function and phenotypes of major human and murine immune cell subsets, superimposed on a multidimensional immune cell population scatter plot. These images are taken from the third edition of EJI’s Flow Cytometry Guidelines by Cossarizza et al., a comprehensive resource prepared … Read more
- Survival of Human Bone Marrow Plasma Cells In Vitro Depends on the Support of the Stromal Cells, PI3K, and Canonical NF‐kappaB Signaling. [[{“value”:”Zehra Uyar‐Aydin,
Shirin Kadler,
Roland Lauster,
Sina Bartfeld,
Mark Rosowski”}]]by inmunoadminThis study established a 21-day in vitro culture system for human long-lived plasma cells (LLPCs), demonstrating their survival and antibody secretion depend on stromal cell contact. PI3K and NF-kappaB pathways were crucial for LLPC survival, aligning with findings in mice. This model advances the understanding of human LLPC biology. ABSTRACT Contrary to short-lived plasma cells, … Read more
- Pathogenic and Nonpathogenic Antibody Responses in Allergic Diseases. [[{“value”:”Marc Ehlers,
Friederike Jönsson”}]]by inmunoadminAllergen-specific antibodies can be detected in a large proportion of individuals. Still, only a fraction suffer from clinical signs of allergic disease, and of these, some, but not all, will develop more severe forms of the disease over time or respond to allergen-specific immunotherapy. Here, we review the concept of nonpathogenic versus pathogenic antibody responses … Read more
- Ubiquitin‐Conjugating Enzyme Ubc13 in Macrophages Suppresses Lung Tumor Progression Through Inhibiting PD‐L1 Expression. [[{“value”:”Siying Sun,
Jun Ni,
Jiamin Liu,
Juofang Tan,
Runsen Jin,
Hecheng Li,
Xuefeng Wu”}]]by inmunoadminSun et al. demonstrate that deficiency of macrophage-intrinsic Ubc13 increases Akt activation and PD-L1 expression, which triggers macrophage protumoral polarization and lung tumor progression. Ubc13-depletion in macrophage also increases infiltration of myeloid-derived suppressor cells and decreases proliferative and effector function of CD8+ T lymphocytes in the tumor microenvironment. ABSTRACT Tumor cell-intrinsic ubiquitin-conjugating enzyme Ubc13 promotes tumorigenesis, … Read more
- Researcher Reflections—Inspiring Paths in Academia. [[{“value”:”Annika Hausmann,
Ioana Sandu,
Rami Bechara,
Liv Eidsmo,
Adrian Liston,
Shruti Naik,
Charlotte L. Scott,
Matteo Villa,
Eduardo Villablanca,
Natalia Muñoz‐Wolf”}]]by inmunoadminABSTRACT The journey of early-career researchers (ECRs) in immunology is marked by many challenges but also exciting prospects. Here we bring a snapshot of the recent ECR workshop organized by the Young European Federation of Immunology Societies (yEFIS) during the 7th European Congress of Immunology (ECI), which took place in Dublin Ireland from September 1 … Read more
- B Cell Receptor Repertoire Analysis of the CD21lo B Cell Compartment in Healthy Individuals, Patients With Sjögren’s Disease, and Patients With Radiographic Axial Spondyloarthritis. [[{“value”:”Rick Wilbrink,
Linda van der Weele,
Anneke J. P. L. Spoorenberg,
Niek de Vries,
Ilse T. G. Niewold,
Gwenny M. Verstappen,
Frans G. M. Kroese”}]]by inmunoadminThis study demonstrates that CD27−CD21lo B cells harbor a relatively low frequency of somatic mutations in their BCR compared with CD27+CD21lo B cells and early plasmablasts. Clonal relationships were present among all three subsets in both health and disease, indicating that CD27−CD21lo B cells actively contribute to immune responses. ABSTRACT B cells with low or … Read more
- Characterization of Human CD8αβ Interaction With Classical and Unconventional MHC Molecules. [[{“value”:”Ben de Wet,
Robert Alan Simmons,
Richard J. Suckling,
Rita Szoke‐Kovacs,
Salah Mansour,
Marco Lepore,
David K. Cole,
Jakub Jaworski,
Alexandra L. Chapman,
Milos Aleksic”}]]by inmunoadminHuman CD8αβ was produced as an Fc-fusion via knob-in-hole mediated heterodimerization and bound class I pHLA and MR1 with similar affinity to human CD8αα, but did not bind CD1a, b, c, or d. An inverse relationship between CD8:pHLA affinity and TCR:pHLA was observed for several alleles. ABSTRACT The CD8 co-receptor exists as both an αα … Read more
- Half‐Life Extension of the IgG‐Degrading Enzyme (IdeS) Using Fc‐Fusion Technology. [[{“value”:”Victoria Daventure,
Melissa Bou‐Jaoudeh,
Emna Hannachi,
Alejandra Reyes‐Ruiz,
Amélia Trecco,
Sandrine Delignat,
Sébastien Lacroix‐Desmazes,
Claire Deligne”}]]by inmunoadminWe engineered the IgG-degrading enzyme IdeS as a Fc-fusion protein. IdeS-Fc exhibits similar hydrolysing efficacy to its native counterpart, but has an increased half-life, translating into prolonged therapeutic degradation of IgG. ABSTRACT Imlifidase (IdeS) is a bacterial protease that hydrolyzes human IgG in their hinge region, decreasing their half-life and abrogating their Fc-mediated properties. It … Read more
- The Efficiency of Brain‐Derived Neurotrophic Factor Secretion by mRNA‐Electroporated Regulatory T Cells Is Highly Impacted by Their Activation Status. [[{“value”:”Jasper Van den Bos,
Ibo Janssens,
Morgane Vermeulen,
Amber Dams,
Hans De Reu,
Stefanie Peeters,
Carole Faghel,
Yousra El Ouaamari,
Inez Wens,
Nathalie Cools”}]]by inmunoadminFollowing isolation and expansion, the function of Tregs can be enhanced through mRNA electroporation, resulting in protein overexpression. Our findings reveal that protein secretion post-electroporation is dependent on the activation state of the Tregs. Specifically, only activated Tregs were capable of successfully secreting BDNF after BDNF-mRNA electroporation. ABSTRACT Genetic engineering of regulatory T cells (Tregs) … Read more
- BAFF Blockade Attenuates B Cell MALT Formation in Conditional Nlrc5‐Deficient Mice With Helicobacter felis Infection. [[{“value”:”Dongmei Tong,
Yuqi He,
Shambel Araya Haile,
Zoe Lee,
Lena H. M. Le,
Jack Emery,
Georgie Wray‐McCan,
Michelle Chonwerawong,
Dana J. Philpott,
Paul J. Hertzog,
Pascal Schneider,
Richard L. Ferrero,
Le Ying”}]]by inmunoadminBAFF blockade suppresses B cell MALT formation in H. felis-infected Nlrc5mø-KO mice. The upregulation of protein-coding BAFF transcripts in NLRC5-deficient macrophages suggests a novel NLRC5–BAFF signalling axis. ABSTRACT Helicobacter infection is a key cause of gastric B cell mucosa–associated lymphoid tissue (MALT) lymphoma. This study examined the role of B cell–activating factor (BAFF), a major … Read more
- TOX Does Not Drive Sepsis‐Induced T‐Cell Exhaustion. [[{“value”:”Yingyu Qin,
Yilin Qian,
Shengqiu Liu,
Rong Chen”}]]by inmunoadminThe number of exhausted T cells continuously increases in chronic sepsis. Both knockout and “knockdown” of TOX failed to alleviate sepsis-induced T-cell exhaustion. However, deletion of TOX impaired the effector function of T cells, suggesting that TOX is not involved in driving T-cell exhaustion in sepsis. ABSTRACT The immune system undergoes profound dysregulation in sepsis, … Read more
- KIRA6 is an Effective and Versatile Mast Cell Inhibitor of IgE‐mediated Activation. [[{“value”:”Veronika Wunderle,
Thomas Wilhelm,
Shatha Boukeileh,
Jonas Goßen,
Michael A. Margreiter,
Roman Sakurov,
Sandro Capellmann,
Maike Schwoerer,
Nabil Ahmed,
Gina Bronneberg,
Michel Arock,
Christian Martin,
Thomas Schubert,
Francesca Levi‐Schaffer,
Giulia Rossetti,
Boaz Tirosh,
Michael Huber”}]]by inmunoadminWe discovered that the IRE1α−targeting small molecule KIRA6 is a potent inhibitor of the SRC family kinases LYN and FYN. Thereby it prevents mast cell activation by blocking the antigen-triggered activation of the high affinity IgE receptor FcεRI. Thus, KIRA6 is able to abolish mast cell-mediated allergic responses. ABSTRACT Mast cell (MC)-driven allergic diseases are … Read more
- Distinct Tissue‐Dependent Composition and Gene Expression of Human Fetal Innate Lymphoid Cells. [[{“value”:”Inga E. Rødahl,
Martin A. Ivarsson,
Liyen Loh,
Jeff E. Mold,
Magnus Westgren,
Danielle Friberg,
Jenny Mjösberg,
Niklas K. Björkström,
Nicole Marquardt,
Douglas F. Nixon,
Jakob Michaëlsson”}]]by inmunoadminCD16+ and CD16− Natural Killer cells and type 3 innate lymphoid cells are found in early human development with distinct distribution and phenotype across fetal tissues. The presence of CD34+RORyt+Eomes± cells selectively in the fetal intestine indicates the presence of tissue-specific progenitors. ABSTRACT The human fetal immune system starts to develop in the first trimester … Read more
- Activation of STAT6 in Intestinal Epithelial Cells Predisposes to Gut Inflammation. [[{“value”:”Stefanie Westermann,
Daniel Radtke,
Lisa Kramer,
Stefan Wirtz,
David Voehringer”}]]by inmunoadminExpression of constitutively active STAT6 in IECs of VillinCre_STAT6vt mice leads to enhanced pathology during DSS-induced colitis by regulation of genes involved in various functional pathways associated with epithelial barrier integrity in the colon. ABSTRACT Ulcerative colitis (UC) is an inflammatory bowel disease (IBD) often associated with a Type 2 immune response. Although previous reports … Read more
- Human Genetic GLUT1 Deficiency Results in Impaired T Cellular IFN‐γ Production. [[{“value”:”Renske de Jong,
Anandhi Rajendiran,
Judit Turyne Hriczko,
Sudheendra Hebbar Subramanyam,
Alina Rein,
Martin Häusler,
Thorsten Orlikowsky,
Norbert Wagner,
Daniel Erny,
Kim Ohl,
Klaus Tenbrock”}]]by inmunoadminGLUT1 deficiency prevents glucose uptake in T cells resulting in lower intracellular ATP generation and IFNy production.
- SHIP‐1 Differentially Regulates IgE‐Induced IL‐10 and Antiviral Responses in Human Monocytes. [[{“value”:”Siva Kumar Solleti,
Bailey E. Matthews,
Jingyi Wu,
Regina K. Rowe”}]]by inmunoadminIgE-mediated activation of human monocytes results in SHIP-1 activation, IL-10 production, and inhibition of antiviral IP-10. SHIP-1 inhibition by 3AC enhanced IL-10 but suppressed antiviral IP-10. This indicates differential regulatory roles for SHIP-1, which could impact antiviral responses in allergic diseases. ABSTRACT IgE-mediated stimulation of monocytes regulates multiple cellular functions including cellular maturation, cytokine release, … Read more
- Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC. [[{“value”:”Diana Dudziak,
Lukas Heger,
William W Agace,
Joyce Bakker,
Tanja D. de Gruijl,
Regine J. Dress,
Charles‐Antoine Dutertre,
Thomas M. Fenton,
Marieke F. Fransen,
Florent Ginhoux,
Oded Heyman,
Yael Horev,
Florian Hornsteiner,
Vinitha Kandiah,
Paz Kles,
Ruth Lubin,
Gabriel Mizraji,
Anastasia Prokopi,
Or Saar,
Sieghart Sopper,
Patrizia Stoitzner,
Helen Strandt,
Martina M Sykora,
Elisa C. Toffoli,
Christoph H. Tripp,
Kim van Pul,
Rieneke van de Ven,
Asaf Wilensky,
Simon Yona,
Claudia Zelle‐Rieser”}]]by inmunoadminWe here provide detailed protocols for isolating single cell suspension from human nonlymphoid tissues, such as gingiva, lung, intestine, skin, and different tumor tissues as well as tumor-draining lymph nodes. Further, we provide gating strategies for flow cytometric analysis of human DC subpopulations in diverse nonlymphoid tissues. Abstract This article is part of the Dendritic … Read more
- A Switch Protein Adapter for Anti‐LILRB4 CAR‐T Cells. [[{“value”:”Ryan Huang,
Heyu Chen,
Jingjing Xie,
Qi Lou,
Lingxiao Tan,
Ningyan Zhang,
Zhiqiang An,
Samuel John,
Cheng Cheng Zhang”}]]by inmunoadminChimeric antigen receptor (CAR) T-cells have proven effective in hematologic malignancies targeting a restricted set of tumor-associated antigens. In this work, we demonstrate that CAR-T cells can be redirected to alternative tumor antigens through a switch protein adapter with antitumor efficacy in preclinical models. ABSTRACT Chimeric antigen receptor-T cell (CAR-T) immunotherapy has shown remarkable results … Read more
- Dual Activation‐Induced Marker Combinations Efficiently Identify and Discern Antigen‐Specific and Bystander‐Activated Human CD4+ T Cells. [[{“value”:”Maria Grazia Ceraolo,
Maristella Leccese,
Antonino Cassotta,
Sara Triolo,
Mauro Bombaci,
Elena Coluccio,
Daniele Prati,
Riccardo Ungaro,
Sergio Abrignani,
Alessandra Bandera,
Federica Sallusto,
Antonio Lanzavecchia,
Samuele Notarbartolo”}]]by inmunoadminIdentifying activated T lymphocytes and discerning antigen-specific from bystander T cells is crucial for understanding immune responses. Dual AIM combinations significantly enhance the ability to distinguish antigen-specific from bystander-activated T cells compared with single AIMs. They also have a better positive predictive value and specificity with a modest reduction in sensitivity. ABSTRACT Identifying activated T … Read more
- Tumor‐Expressed SPPL3 Supports Innate Antitumor Immune Responses. [[{“value”:”Tamara Verkerk,
Antonius A. de Waard,
Sofie J. I. Koomen,
Jasper Sanders,
Tineke Jorritsma,
Anouk T. Pappot,
Nordin D. Zandhuis,
Tao Zhang,
Manfred Wuhrer,
Arie J. Hoogendijk,
Floris P. J. van Alphen,
Maartje van den Biggelaar,
Hannes S. J. Stockinger,
Klaas P. J. M. van Gisbergen,
Robbert M. Spaapen,
S. Marieke van Ham”}]]by inmunoadminTumor and immune cells interact through receptor–ligand interactions. B3GNT5 produces nsGSL on tumor cells which is regulated by SPPL3. When SPPL3 is absent, elevated nsGSL levels hinder receptor–ligand binding, leading to reduced immune cell activation. ABSTRACT The development of an effective antitumor response relies on the synergistic actions of various immune cells that recognize tumor … Read more
- Tviblindi algorithm identifies branching developmental trajectories of human B‐cell development and describes abnormalities in RAG‐1 and WAS patients. [[{“value”:”Marina Bakardjieva,
Ondřej Pelák,
Marjolein Wentink,
Hana Glier,
David Novák,
Jitka Stančíková,
Daniela Kužílková,
Ester Mejstříková,
Iga Janowska,
Marta Rizzi,
Mirjam van der Burg,
Jan Stuchlý,
Tomáš Kalina”}]]by inmunoadminNovel computational framework tviblindi identifies branching B cell developmental trajectories in healthy (HD) and abnormal (RAG-1 and Wiskott–Aldrich syndrome primary immunodeficiency) bone marrow and peripheral blood samples using 30 parameter mass cytometry. Integrated vaevictis projection allows interpretation of trajectories to κ (grey arrow) and λ (black arrow) light chain expressing B cells, memory development branching … Read more
- MicroRNA‐146a deficiency enhances host protection against murine cytomegalovirus. [[{“value”:”Pamela Wong,
Jeffrey W. Leong,
Hyogon Sohn,
Lily Chang,
Catherine R. Keppel,
Carly C. Neal,
Celia C. Cubitt,
Tony Yao,
Molly P. Keppel,
Jennifer Tran,
Allison Burdi,
Kimberly Hwang,
Leslie A. Fogel,
Timothy Schappe,
Lynne Marsala,
Melissa M. Berrien‐Elliott,
Julia A. Wagner,
Stephanie E. Schneider,
Ryan P. Sullivan,
Jeanette T. Pingel,
Megan A. Cooper,
Anthony R. French,
Todd A. Fehniger”}]]by inmunoadminMiR-146a−/− mice are protected from lethal MCMV infection. This protection is dependent on miR-146a expression in the hematopoietic compartment and NK cells. miR-146a−/− mice have increased liver mature NK cells, STAT-1 phosphorylation, and Ly49H+ NK cell and T-cell expansion, which can contribute to protecting the host from lethal MCMV. Abstract Natural killer (NK) cells are … Read more
- Probing TCR Specificity Using Artificial In Vivo Diversification of CDR3 Regions. [[{“value”:”Orlando B. Giorgetti,
Annette Haas‐Assenbaum,
Thomas Boehm”}]]by inmunoadminDiversification of transgenic TCRs by CRISPR/Cas9-mediated mutagenesis of Tcra and Tcrb chain genes in vivo converts monoclonal repertoires of known specificity into an oligoclonal pool of TCRs of altered antigen reactivities. This generally applicable approach helps to identify crucial amino acid residues in the CDR3 regions required for antigen recognition. ABSTRACT The T-cell receptor sequences … Read more
- Function and Spatial Organization of Tumor‐Invasive Human γδ T Cells—What Do We Know?. [[{“value”:”Kilian Wistuba‐Hamprecht,
Hans‐Heinrich Oberg,
Daniela Wesch”}]]by inmunoadminThis review provides deeper insights into the localization of intra-tumoral γδ T lymphocytes in solid tumors. Additionally, a pro- or anti-tumoral influence of the tumor microenvironment and expression of checkpoint inhibitory molecules on γδ T cell functionality is described. To overcome the suppression of tumor-infiltrating γδ T cells, future immunotherapy approaches are summarized. ABSTRACT Human … Read more
- IL1R2 Acts as a Negative Regulator of Monocyte Recruitment During Inflammation. [[{“value”:”Adeline Cros,
Elodie Segura”}]]by inmunoadminIl1r2 deficiency in monocytes dysregulates their trafficking to inflamed tissues through increased production of the monocyte chemoattractant CCL2. ABSTRACT IL1-β plays a central role in inflammation but its biological action needs to be tightly controlled. Such negative regulation can be exerted by the decoy receptor IL1R2. However, IL1R2 biology in immune cells remains poorly characterized, … Read more
- Distinct Requirements for CD4+ T Cell Help for Immune Responses Induced by mRNA and Adenovirus‐Vector SARS‐CoV‐2 Vaccines. [[{“value”:”Lyn Yong,
Claire Hutchings,
Eleanor Barnes,
Paul Klenerman,
Nicholas M. Provine”}]]by inmunoadminThe role of CD4+ T cells in promoting cellular and humoral immunity induced by novel mRNA–LNP and adenovirus vector vaccine technologies is incompletely understood. Surprisingly, we identified a discordant need for CD4+ T cell help based on both vaccine platform and arm of adaptive immunity examined. These data provide further evidence of the unique biology … Read more
- Differential Characteristics on TCR Embedding Landscape Among Immune Checkpoint Blockade Responders Versus Non‐Responders Against Non‐Small Cell Lung Carcinoma. [[{“value”:”Peiling Tsou,
Chang‐Jiun Wu”}]]by inmunoadminFor each patient in the NSCLC cohort, TCR sequences were converted into numerical vectors using the TCR2Vec model and projected onto a 2D landscape. The aggregated fractions of anti-LAA TEGs per patient were calculated. ICB responders had over-represented anti-LAA TEGs compared to non-responders.
- Crafting an MSCA PhD Masterpiece: Guiding Students on the Verge of Discovery. [[{“value”:”Lisa Dratva,
Alice Driessen,
Igor Filippov,
Stéphane Guillaume,
Lisa Hoenicke,
Jean‐Christophe Lone”}]]by inmunoadminEuropean Journal of Immunology, EarlyView.
- Memory Phenotype Tfh Cells Develop Without Overt Infection and Support Germinal Center Formation and B Cell Responses to Viral Infection. [[{“value”:”Alistair L. J. Symonds,
Zabreen Busharat,
Mengmeng Du,
Tizong Miao,
Suling Li,
Xiujuan Hou,
Ping Wang”}]]by inmunoadminIn the steady state, naïve CD4 T cells can develop into memory phenotype (MP) cells. We discovered that MP T cells include a subpopulation of Tfh cells. MP Tfh cells share key markers with pathogen-induced memory Tfh cells and can support germinal center formation and antibody responses to infection. ABSTRACT Pathogen-induced memory Tfh cells are … Read more
- Metabolic Reprogramming of Fibroblastic Reticular Cells in Immunity and Tolerance. [[{“value”:”Dejun Kong,
Marina WillsonShirkey,
Wenji Piao,
Long Wu,
Shunqun Luo,
Allision Kensiski,
Jing Zhao,
Young Lee,
Reza Abdi,
Hong Zheng,
Jonathan S. Bromberg”}]]by inmunoadminThis review addresses how fibroblastic reticular cells (FRCs) undergo metabolic reprogramming in response to infections, autoimmune diseases, and cancer, adjusting their glycolysis, oxidative phosphorylation, and fatty acid metabolism to modulate immune functions. These metabolic shifts in FRCs highlight potential therapeutic strategies to improve immune regulation across various disease contexts. ABSTRACT Fibroblastic reticular cells (FRCs) are … Read more
- Cytokine Autoantibodies Alter Gene Expression Profiles of Healthy Donors. [[{“value”:”Jakob Hjorth von Stemann,
Florian Dubois,
Violaine Saint‐André,
Vincent Bondet,
Celine Posseme,
Bruno Charbit,
Lluis Quintana‐Murci,
Morten Bagge Hansen,
Sisse Rye Ostrowski,
Darragh Duffy,
Milieu Intérieur Consortium”}]]by inmunoadminWorking on the 1000-donor Milieu Interieur cohort with ex vivo whole blood stimulation, we demonstrate that naturally occurring cytokine autoantibodies (c-aAb) in healthy donors can neutralize cytokine availability and regulate proinflammatory gene expression. ABSTRACT Autoantibodies against cytokines (c-aAb) have been implicated in the pathophysiology of autoimmune diseases, and a variety of infections. In addition, several … Read more
- The Microbiome Modifies Manifestations of Hemophagocytic Lymphohistiocytosis in Perforin‐Deficient Mice. [[{“value”:”Jasmin Mann,
Solveig Runge,
Christoph Schell,
Katja Gräwe,
Gudrun Thoulass,
Jessica Lao,
Sandra Ammann,
Sarah Grün,
Christoph König,
Sarah A. Berger,
Benedikt Hild,
Peter Aichele,
Stephan P. Rosshart,
Stephan Ehl”}]]by inmunoadminThis study investigates the microbiome as a potential factor underlying the apparent differences in immune stimulation required for HLH induction in perforin-deficient mice compared to FHL-2 patients. Using PKO-Wildlings, we show that while immune activation by natural microbiota is not sufficient to induce HLH, it can influence manifestations of LCMV-induced disease. ABSTRACT Primary hemophagocytic lymphohistiocytosis … Read more
- Neural Crest‐Derived Mesenchymal Cells Support Thymic Reconstitution After Lethal Irradiation. [[{“value”:”Doris Narki Tetteh,
Kana Isono,
Mari Hikosaka‐Kuniishi,
Hidetoshi Yamazaki”}]]by inmunoadminThymic NC-derived mesenchymal cells produce factors, including Flt3L and Periostin, after irradiation. Depletion of NC-derived mesenchyme reduces these factors, decreases the abundance of ETPs, and impairs thymic reconstitution postirradiation. The addition of Flt3L and Periostin after NC-derived mesenchyme depletion restores reconstitution, highlighting the importance of NC-derived mesenchyme in thymus regeneration. ABSTRACT Reconstitution of the thymus … Read more
- Type I Interferon Drives a Cellular State Inert to TCR‐Stimulation and Could Impede Effective T‐Cell Differentiation in Cancer. [[{“value”:”Dillon Corvino,
Martin Batstone,
Brett G. M Hughes,
Tim Kempchen,
Susanna S Ng,
Nazhifah Salim,
Franziska Schneppenheim,
Denise Rommel,
Ananthi Kumar,
Sally Pearson,
Jason Madore,
Lambross T. Koufariotis,
Lisa Maria Steinheuer,
Dilan Pathirana,
Kevin Thurley,
Michael Hölzel,
Nicholas Borcherding,
Matthias Braun,
Tobias Bald”}]]by inmunoadminMultimodal single-cell RNA sequencing identified a population of CD8+ T cells elevated in tumor tissue enriched for interferon-stimulated genes (ISG). These ISG cells are clonally related to a population of Granzyme K (GZMK)-expressing CD8+ T cells. However, unlike GZMK-expressing T cells, ISG cells were transcriptionally inert in response to in vitro stimulation. Created in BioRender.com … Read more
- B Cells With Complementary B Cell Receptors Can Kill Each Other. [[{“value”:”Ramakrishna Prabhu Gopalakrishnan,
Marius Sigurdsson Østrøm,
Frode Miltzow Skjeldal,
Oddmund Bakke,
Bjarne Bogen,
Peter Csaba Huszthy”}]]by inmunoadminDepending on whether the interactions occur in vitro or in vivo, physical binding between two B cells with complementary BCRs leads to distinct outcomes for the participating cells. In vitro, both the Id+ B cell and the complementary anti-idiotypic B cell undergo apoptosis. In vivo, follicular αId B cells expand, whereas marginal zone Id+ B … Read more
- Characterization of the Molecular Signature of Human Monocytes in Aging and Myelodysplastic Neoplasms. [[{“value”:”Christina Maria Rimpa,
Maria Grigoriou,
Athanasios Tasis,
Nikolaos Paschalidis,
Anastasia Filia,
Giannis Vatsellas,
Panagiotis Papazoglou,
Antonios Chatzigeorgiou,
Chrysa Kymparidou,
Menelaos Papoutselis,
Christina Misidou,
Theodoros Spyropoulos,
Despoina Dimitriou,
Haralampos Hatzikirou,
Konstantinos Liapis,
Eleftheria Lamprianidou,
Ioannis Kotsianidis,
Ioannis Mitroulis”}]]by inmunoadmin• Aging leads to chronic inflammation and immune dysfunction, heightening the risk of myeloid malignancies like MDS and CMML. • Both aging and MDS show alterations in monocyte subtypes and function. Aging boosts inflammatory genes upregulation, whereas MDS favors antigen presentation, reflecting distinct immune and disease-specific adaptations. • MDS shows reduced inflammatory activity in CD14+ … Read more
- Rise of a CD27− IgD− CD11c+ B cells population in kidney recipients achieving long‐term graft stability under immunosuppression. [[{“value”:”François Brinas,
Nicolas Sailliet,
Gaëlle Tilly,
Laurence Delbos,
Clarisse Kerleau,
Magali Giral,
Nicolas Degauque,
Sophie Brouard,
Richard Danger”}]]by inmunoadminWe performed a comprehensive blood phenotyping in 24 kidney-transplanted patients and 10 healthy volunteers. Among numerous immune cell subsets, mainly differences in B-cell populations were highlighted, with an increase of CD11c+ memory B cells in blood from kidney transplanted patients with long-term posttransplantation time. Abstract The use of immunosuppressive treatment is required to prevent rejection … Read more
- Neutrophil Extracellular Traps Participate in the Pathogenesis of Lupus Through S100A10‐Mediated Regulatory T‐Cell Differentiation and Functional Abnormalities. [[{“value”:”Hua Guo,
Qian Liang,
Zhonghui Xue,
Junling Yang,
Pengyu Chen,
Juan Ji,
Jing Li,
Genkai Guo,
Haixia Cao,
Xiaoqi Sha,
Rui Zhao,
Chen Dong,
Zhifeng Gu”}]]by inmunoadminNeutrophil extracellular traps (NETs) contribute to SLE development, yet their interaction with Regulatory T cells (Tregs) remains obscure. This study reveals that selectively enhanced NETs in mice suppress Treg production and function. Through modulating naive CD4+ T cells via TLR4, NETs inhibit Treg differentiation, bridging innate and adaptive immune responses. ABSTRACT In systemic lupus erythematosus … Read more
- Circulating levels of endogenous complement inhibitors correlate inversely with complement consumption in systemic lupus erythematosus. [[{“value”:”Stef van der Meulen,
Rory C. Monahan,
Kyra A. Gelderman,
Cees van Kooten,
Y.K. Onno Teng,
Tom W.J. Huizinga,
Gerda M. Steup‐Beekman,
Leendert A. Trouw”}]]by inmunoadminComplement activation is a distinct feature of (neuropsychiatric) systemic lupus erythematosus. However, little is known about complement regulation by endogenous soluble complement inhibitors. We show differences in serum levels between (NP)SLE patients and healthy controls, but regulator levels are not discriminative between NPSLE and SLE. Created with Biorender.com. Abstract Systemic lupus erythematosus (SLE) is marked … Read more
- Correct stimulation of CD28H arms NK cells against tumor cells. [[{“value”:”Raphaëlle Leau,
Pierre Duplouye,
Virginie Huchet,
Véronique Nerrière‐Daguin,
Bernard Martinet,
Mélanie Néel,
Martin Morin,
Richard Danger,
Cécile Braudeau,
Régis Josien,
Gilles Blancho,
Fabienne Haspot”}]]by inmunoadminTargeting CD28H with a properly selected antibody activates NK cells by increasing their cytotoxicity and overcoming inhibitory signals. ScRNA-seq analysis of ccRCC shows that CD28H+ NK cells infiltrate HHLA2+ tumors and could be effectively targeted by this antibody thus offering a promising approach for enhancing antitumor responses. Abstract Tumor evasion has recently been associated with … Read more
- Colony stimulating factor 1 receptor (Csf1r) expressing cell ablation in mafia (macrophage‐specific Fas‐induced apoptosis) mice alters monocyte landscape and atherosclerotic lesion characteristics. [[{“value”:”Indira Medina,
Elias B Wieland,
Lieve Temmerman,
Jeroen J.T. Otten,
Beatriz Bermudez,
Ilze Bot,
Timo Rademakers,
Erwin Wijnands,
Leon Schurgers,
Barend Mees,
Theo J.C. van Berkel,
Pieter Goossens,
Erik A.L. Biessen”}]]by inmunoadminHere, we studied the impact of myeloid cell depletion on atherosclerosis in Apoe − /− mice using a Macrophage Fas-induced apoptosis (MaFIA) mouse model. MaFIA mice carry an inducible apoptosis gene under the control of the Csf1r promotor, enabling specific and transient depletion of Csf1r-expressing cells. Upon systemic induction of apoptosis, plaque macrophage and smooth … Read more
- Lymphotoxins from distinct types of lymphoid cells differentially contribute to neuroinflammation. [[{“value”:”Violetta S. Gogoleva,
Marina S. Drutskaya,
Alexander I. Vorontsov,
Kamar‐Sulu N. Atretkhany,
Alexey A. Belogurov Jr.,
Andrey A. Kruglov,
Sergei A. Nedospasov”}]]by inmunoadminT-cell-derived lymphotoxins promoted IL-17A+ and GM-CSF+ responses at disease onset. At peak EAE, ILC3-derived lymphotoxins inhibited the infiltration of IFN-ɣ- and GM-CSF-producing CD4+ T cells into the CNS, while T-cell-derived lymphotoxins limited Treg accumulation. B-cell-derived lymphotoxins promoted type 1 cytokine production by T cells in rhMOG1–125-induced EAE. Abstract Lymphotoxin α and lymphotoxin β (LTs), TNF … Read more
- Perioperative systemic IL‐6 and immune‐adipose‐ metabolism transcription in tumour and tumour adjacent breast cancer. [[{“value”:”Carolyn Cullinane,
Roisin M. Connolly,
Mark Corrigan,
Henry P. Redmond,
Cathriona Foley”}]]by inmunoadminImmune-adipose-metabolism factors in the macro- and micro-breast-tumour environment are influenced by the magnitude of surgery and certain clinicopathological features. These results provide insight into the complex immune processes of the peri-operative period. Abstract Surgical resection is the primary treatment approach for patients with breast cancer. Despite optimal multimodal treatment, metastatic recurrence remains a risk. Surgery-mediated … Read more
- Atg16l2 augments Nlrc4 inflammasome activation by facilitating NAIPs–NLRC4 association. [[{“value”:”Zhoujin Wen,
Tianli Yuan,
Jiamin Liu,
Dongyang Wang,
Jun Ni,
Xuehan Yan,
Jian Tang,
Jiayin Tang,
Xuefeng Wu,
Zheng Wang”}]]by inmunoadminSchematic diagram of ATG16L2-mediated NLRC4 inflammasome activation during Salmonella typhimurium infection. After NAIPs recognize the different components of S. typhimurium, ATG16L2 promotes the binding of NAIPs to NLRC4, enhancing the oligomerisation of ASC and the subsequent caspase-induced cleavage of IL-1β and GSDMD. N-terminal perforates the cell membrane, causing pyroptosis and LDH leakage. Abstract As cytoplasmic … Read more
- Molecular and functional in vivo characterisation of murine Dectin‐1 isoforms. [[{“value”:”Nadja Leinung,
Torben Mentrup,
Sajma Hodzic,
Bernd Schröder”}]]by inmunoadminDectin-1 is a C-type lectin receptor for the innate sensing of fungi. This receptor exists in two major isoforms in mice differing in a stalk domain, which determines their degradative pathway. Here, we characterise the properties of the two isoforms in a cellular context and using a newly established in vivo model. Abstract Dectin-1 is … Read more
- Modulation of the K/BxN arthritis mouse model and the effector functions of human fibroblast‐like synoviocytes by liver X receptors. [[{“value”:”María Jesús Domínguez‐Luis,
Javier Castro‐Hernández,
Sergio Santos‐Concepción,
Ana Díaz‐Martín,
Mayte Arce‐Franco,
Natán Pérez‐González,
Mercedes Díaz,
Antonio Castrillo,
Eduardo Salido,
José David Machado,
Mónica Gumá,
Maripat Corr,
Federico Díaz‐González”}]]by inmunoadminIn the K/BxN arthritis mouse model, Nr1h2/3−/− animals showed exacerbated arthritis, histologic inflammation and joint destruction, and increased synovial metalloproteases, IL-1β and CCL2 in joints compared with wild-type animals. In FLS from RA patients, LXR agonists inhibited cell proliferation and collagenase activity in response to TNF, chemokine-induced migration, and CXCL12 production. Abstract The role of … Read more
- Alveolar epithelial cells shape lipopolysaccharide‐induced inflammatory responses and reprogramming of alveolar macrophages. [[{“value”:”Wei Jiang,
Yeying Chen,
Cheng‐Yun Yu,
Benkun Zou,
Yimeng Lu,
Qian Yang,
Zihui Tang,
Weiying Mao,
Jing Li,
Han Han,
Lingyun Shao,
Jiashun Zeng,
Yiwei Chu,
Jianguo Tang,
Mingfang Lu”}]]by inmunoadminAfter exposure to LPS, Alveolar macrophages (AMs) secrete TNF-α to induce GM-CSF production by alveolar epithelial cells (AECs). GM-CSF promotes AMs to secrete IL-6 and primes AMs for subsequent challenges. These results show that in response to MAMP stimulation, AMs and AECs respond collaboratively and AMs are primed. Abstract Alveolar macrophages (AMs) are sentinels in … Read more
- Immune response to topical sodium lauryl sulfate differs from classical irritant and allergic contact dermatitis. [[{“value”:”Marvin Nüsken,
Fabian Heinemeier,
Silke Sabina Matzke,
Patryk Porebski,
Susann Forkel,
Prasad Dasari,
Andrea Braun,
Andreas Erich Zautner,
Michael Peter Schön,
Timo Buhl”}]]by inmunoadminWe investigated sodium lauryl sulfate (SLS)-skin reactions because of its relevance in patch tests of patients, and we compared its effects with ACD-inducing oxazolone (OXA) and ICD-inducing croton oil (CrO). SLS-induced contact dermatitis differed from classical ACD and ICD, as it elicited less pronounced immune alterations. Abstract Sodium lauryl sulfate (SLS) is used as a … Read more
- 2024 German Society for Immunology Prizes.by inmunoadminEuropean Journal of Immunology, EarlyView.
- The macrophage migration inhibitory factor/CD74 axis in traumatic spinal cord injury: lessons learned from animal and human studies. [[{“value”:”Serina Rubio,
Veerle Somers,
Judith Fraussen”}]]by inmunoadminBoth animal and human studies have indicated the importance of MIF and CD74 in traumatic SCI. This review discusses how the MIF/CD74 axis affects numerous cell types and recovery post-SCI and proposes a new path for investigation focused on B cells. Abstract Traumatic spinal cord injury (SCI) is a severe condition leading to long-term impairment … Read more
- Characterizing dynamic tumor–immune interactions in lung adenocarcinoma through orthotopic allograft modeling. [[{“value”:”Mingjun Shi,
Tianqi Dong,
Jiaming Lin,
Liu Huang,
Huixia Zhang,
Shuguo Sun”}]]by inmunoadminAn orthotopic lung adenocarcinoma allograft model with fluorescently labeled cells was developed to investigate the tumor microenvironment. Results demonstrate that CCL7 promotes M2 macrophage polarization, accelerating tumorigenesis and offering novel preclinical tools as well as potential therapeutic targets for lung cancer immunotherapy. Abstract The major clinical challenge in lung cancer immunotherapy is drug resistance. Therefore, … Read more
- Impact of the adenosine receptor A2BR expressed on myeloid cells on immune regulation during pregnancy. [[{“value”:”Stefanie Dietz,
Janine Hebel,
Jessica Rühle,
Alisha Huff,
Holger K. Eltzschig,
Trim Lajqi,
Christian F. Poets,
Christian Gille,
Natascha Köstlin‐Gille”}]]by inmunoadminThe absence of adenosine receptor A2B on myeloid cells in Adora2B923f/f-LysMCre mice resulted in systemic changes in myeloid and lymphoid cell populations during pregnancy. There was an increased expression of IL-6 and a reduction of MDSC and uNK cells. However, there were only marginal differences in pregnancy outcomes. Abstract During pregnancy, the maternal immune system … Read more
- Decoding complement: Novel disease insights and therapeutic horizons. [[{“value”:”Christian M. Karsten,
Jörg Köhl”}]]by inmunoadminEuropean Journal of Immunology, EarlyView.
- CD24 regulates liver immune response and ameliorates acute hepatic injury through controlling hepatic macrophages. [[{“value”:”Jian Zheng,
Jun Xiao,
Yatong Fan,
Honggang Zheng,
Hongyu Liu,
Jie Xiang,
Lei Hai,
Yan Wang,
Xuejun Zhang”}]]by inmunoadminCD24 on hepatic macrophages limits liver injury by interacting with Siglec-G and inhibiting inflammatory pathways. CD24 deficiency exacerbates liver damage, underscoring its potential as a therapeutic target for liver diseases. Abstract Liver injury releases danger-associated molecular patterns, which trigger the immune response. CD24 negatively regulates the immune response by binding with danger-associated molecular patterns, but … Read more
- Blood transcriptome profiling reveals distinct gene networks induced by mRNA vaccination against COVID‐19. [[{“value”:”Lennart Riemann,
Leonie M. Weskamm,
Leonie Mayer,
Ivan Odak,
Swantje Hammerschmidt,
Inga Sandrock,
Michaela Friedrichsen,
Inga Ravens,
Janina Fuss,
Gesine Hansen,
Marylyn M. Addo,
Reinhold Förster”}]]by inmunoadminUsing an unsupervised weighted gene correlation analysis on blood transcriptomes pre- and post-COVID-19 mRNA vaccination, we identify distinct gene networks that are up- and downregulated in response to vaccination. A particular interferon-associated network significantly correlated with induced antibody levels and was further characterized, for example, by identifying network hub genes. Abstract Messenger RNA (mRNA) vaccines … Read more
- IL‐17A‐producing γδ T cells: A novel target in stroke immunotherapy. [[{“value”:”Marius Piepke,
Alina Jander,
Nicola Gagliani,
Mathias Gelderblom”}]]by inmunoadminIn stroke, γδ T cells orchestrate the postischemic immune response in the CNS through their IL-17A production. Notably, the intestinal microbiota and the diet influence IL-17A levels in γδ T cells, offering the potential to modulate γδ17 T cells in the postischemic CNS and to improve stroke outcome. Abstract The activation of the immune system … Read more
- Bridging the gap: Insights in the immunopathology of Lyme borreliosis. [[{“value”:”Marijn E. Snik,
Noor E.I.M. Stouthamer,
Joppe W. Hovius,
Melissa M.J. van Gool”}]]by inmunoadminLyme borreliosis is caused by Borrelia burgdorferi sensu lato transmitted by Ixodes ticks. The disease progresses from early localized infection to more severe conditions driven by ongoing inflammation. Understanding the role of Th1/Th2 balance, B-cell dysfunction, and autoimmunity in Lyme borreliosis pathogenesis could improve diagnosis and treatment strategies. Abstract Lyme borreliosis (LB), caused by Borrelia … Read more
- PP2A catalytic subunit alpha is critically required for CD8+ T‐cell homeostasis and antibacterial responses. [[{“value”:”Xian Zhou,
Meilu Li,
Minji Ai,
Yanfeng Li,
Xingxing Zhu,
Michael J. Hansen,
Jun Zhong,
Kenneth L. Johnson,
Roman Zenka,
Akhilesh Pandey,
Larry R. Pease,
Hu Zeng”}]]by inmunoadminGraphical Abstract: Using a genetic model lacking PP2A catalytic subunit alpha (PP2A Cα) specifically in T cells, we demonstrated that PP2A Cα is essential for maintaining CD8+ T-cell homeostasis and for their antibacterial response. Proteomics analyses revealed multiple pathways regulated by PP2A Cα, including mTORC1, PI3K/AKT, and Jak-STAT5. Abstract Although the functions of tyrosine phosphatases … Read more
- CCAAT/enhancer‐binding protein α‐dependent regulation of granule formation in mast cells by intestinal bacteria. [[{“value”:”Ayaka Iketani,
Mai Takano,
Kazumi Kasakura,
Miono Iwatsuki,
Ayu Tsuji,
Kou Matsuda,
Remina Minegishi,
Akira Hosono,
Yusuke Nakanishi,
Kyoko Takahashi”}]]by inmunoadminSpecific gut microbes suppress granule formation in mast cells by inhibiting serglycin (SRGN) and mast cell protease 4 (Mcpt4) expression via reduced C/EBPα gene methylation, which leads to reduced histamine release. These mechanisms may play a part of antiallergic effects of gut microbiota. Abstract The antiallergic effects of gut microbiota have been attracting attention in … Read more
- TGF‐β1 impairs IgA class switch recombination and production in porcine Peyer’s patches B cells. [[{“value”:”Caiying Wang,
Yue Zhang,
Yabin Lu,
Xin Huang,
Huazheng Jiang,
Guohui Chen,
Yongheng Shao,
Huub F.J. Savelkoul,
Christine A. Jansen,
Guangliang Liu”}]]by inmunoadminBy analyzing the downstream signaling pathways in IgM+ B cells isolated from porcine Peyer’s patches after exogenous TGF-β1 stimulation, we found that TGF-β1 reduces IgA CSR and production in pigs by inhibiting the phosphorylation of p38-MAPK, which may be related to the regulation of TGF-β1 on B-cell activation. Abstract Secretory IgA is crucial for preventing … Read more
- Med1 controls thymic T‐cell migration into lymph node through enhancer‐based Foxo1‐Klf2 transcription program. [[{“value”:”Ning Yuan,
Yanhong Su,
Yang Gao,
Biao Yang,
Tianzhe Zhang,
Qianhao Wang,
Dan Zhang,
Lin Shi,
Anjun Jiao,
Lei Lei,
Lina Sun,
Baojun Zhang”}]]by inmunoadmin(1) Med1 deletion in T cells leads to impaired egress of thymic single-positive cells. (2) Med1 deletion impairs mature T cells’ ability to enter lymph nodes. (3) Med1 promotes Klf2 transcription through facilitating Foxo1 binding to the Klf2 enhancer. Abstract The migration is the key step for thymic T cells to enter circulation and then … Read more
- Immune response to cold exposure: Role of γδ T cells and TLR2‐mediated inflammation. [[{“value”:”Daniel Vasek,
Peter Holicek,
Frantisek Galatik,
Anna Kratochvilova,
Bianka Porubska,
Veronika Somova,
Natalie Fikarova,
Michaela Hajkova,
Martin Prevorovsky,
Jitka M Zurmanova,
Magdalena Krulova”}]]by inmunoadminOur study investigates how short-term and long-term mild cold exposure in rats and regular cold swimmers influences immune response and thermogenesis, highlighting the crucial role of γδ T cells under physiological conditions and TLR2-mediated inflammation. These findings advance our understanding of the complex interactions between thermogenesis, neural signaling, and immune regulation. Abstract The mammalian body … Read more
- Tridecylcyclohexane in incomplete Freund’s adjuvant is a critical component in inducing experimental autoimmune diseases. [[{“value”:”Norifumi Iijima,
Tomoya Hayashi,
Masaaki Niino,
Yoichi Miyamoto,
Masahiro Oka,
Ken J Ishii”}]]by inmunoadminAutoantigen and heat-killed Mycobacterium tuberculosis emulsified in incomplete Freund’s adjuvant (IFA) are capable of inducing numerous autoimmune diseases in animal models. However, the role of IFA components in triggering these diseases remains unknown. In this study, the emulsification with tridecylcyclohexane was critical for the pathogenesis of autoimmune diseases in mice. Abstract Incomplete Freund’s adjuvant (IFA) … Read more
- RAG1/2 induces double‐stranded DNA breaks at non‐Ig loci in the proximity of single sequence repeats in developing B cells. [[{“value”:”Katarina Ochodnicka‐Mackovicova,
Michal Mokry,
Martin Haagmans,
Ted E. Bradley,
Carel J.M. van Noesel,
Jeroen E.J. Guikema”}]]by inmunoadminRAG1/2 endonuclease induces off-target DNA breaks in developing B cells, highlighting its genotoxic potential beyond the immunoglobulin loci. This genome-wide study in mouse pre-B cells identifies RAG1/2-dependent DSBs and uncovers their predominant association with GA and CA dinucleotide repeats and GC-rich motifs, providing new insights into RAG1/2-targeting. Abstract In developing B cells, V(D)J gene recombination … Read more
- Regulation of IFN‐γ production by ZFP36L2 in T cells is time‐dependent. [[{“value”:”Nordin D. Zandhuis,
Aurélie Guislain,
Abeera Popalzij,
Sander Engels,
Branka Popović,
Martin Turner,
Monika C. Wolkers”}]]by inmunoadminWe uncovered a novel time-dependent role for the RNA-binding protein ZFP36L2 in regulating IFN-γ production. ZFP36L2 was redundant in regulating cytokine production during early T-cell activation. However, upon continued T-cell activation, ZFP36L2 dampens the production of IFN-γ by binding to AU-rich elements in the 3ʹUTR and promoting mRNA degradation. Abstract CD8+ T cells kill target … Read more
- Functional Fc receptors are crucial in antibody‐mediated protection against cytomegalovirus. [[{“value”:”Anna Bootz,
Nina Reuter,
Falk Nimmerjahn,
William J. Britt,
Michael Mach,
Thomas H. Winkler”}]]by inmunoadminThe mechanisms of protection of therapeutically applied antibodies in cytomegalovirus infection are so far unknown. Here it is shown in an in vivo mouse model that Fc-receptors are essential for protection from a lethal infection in immunodeficient hosts. Whereas NK cells have a limited role, monocytes are involved in protection. Created with BioRender.com. Abstract Human … Read more
- Site‐specific serology unveils cross‐reactive monoclonal antibodies targeting influenza A hemagglutinin epitopes. [[{“value”:”Philipp C. G. Paparoditis,
Alexander Fruehwirth,
Kajetana Bevc,
Jun Siong Low,
Josipa Jerak,
Laura Terzaghi,
Mathilde Foglierini,
Blanca Fernandez,
David Jarrossay,
Davide Corti,
Federica Sallusto,
Antonio Lanzavecchia,
Antonino Cassotta”}]]by inmunoadminThe authors developed a novel blocking of binding (BoB)-ELISA to accurately estimate the titers of serum antibodies targeting distinct influenza hemagglutinin (HA) epitopes. Leveraging BoB-ELISA, the authors found that serum antibodies targeting the HA head epitopes were immunodominant, whereas antibodies against the stem epitope, although subdominant, were highly prevalent in healthy donors. Importantly, BoB-ELISA allowed … Read more
- Highly sensitive reporter cell line for detection of interferon types I–III and their neutralization by antibodies. [[{“value”:”Kevin Groen,
Roger Kuratli,
Lauren Sar,
Andri Vasou,
Michael Huber,
David J. Hughes,
Benjamin G. Hale”}]]by inmunoadminInterferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity … Read more
- A20 intrinsically influences human effector T‐cell survival and function by regulating both NF‐κB and JNK signaling. [[{“value”:”Gina Dabbah‐Krancher,
Allison Ruchinskas,
Melissa A. Kallarakal,
Katherine P. Lee,
Bradly M. Bauman,
Benjamin Epstein,
Hongli Yin,
Daniel Krappmann,
Brian C. Schaefer,
Andrew L. Snow”}]]by inmunoadminThe ubiquitin-editing enzyme A20 regulates innate and adaptive immune receptor signaling, but its intrinsic function in human T cells is poorly understood. We show that A20 deletion enhances cytokine secretion and alters apoptosis sensitivity in primary human T cells by modulating NF-kB and JNK/AP-1 signaling downstream of the CARD11-BCL10-MALT1 complex. Abstract A20 is a dual-function … Read more
- Chronic stimulation desensitizes β2‐adrenergic receptor responses in natural killer cells. [[{“value”:”Martin Jürgens,
Maren Claus,
Sabine Wingert,
Jens Alexander Niemann,
Lea Katharina Picard,
Elisabeth Hennes,
Ina Haasler,
Birte Hellwig,
Nina Overbeck,
Jörg Reinders,
Jörg Rahnenführer,
Michaela Schedel,
Silvia Capellino,
Carsten Watzl”}]]by inmunoadminβ2-adrenergic receptor (β2AR) stimulation of NK cells inhibits inside-out signaling and LFA-1 activation, explaining the mobilization of NK cells upon acute stress. Repeated or sustained β2AR stimulation desensitizes β2AR via G-protein switch. Local administration of long-acting β2-agonists does not interfere with systemic NK cell functions in asthma patients. Abstract Adrenergic receptors (ARs) are preferentially expressed … Read more
- Liver type 1 innate lymphoid cells undergo apoptosis in murine models of macrophage activation syndrome and are dispensable for disease. [[{“value”:”Amber De Visscher,
Marte Vandeput,
Jessica Vandenhaute,
Bert Malengier‐Devlies,
Eline Bernaerts,
Kourosh Ahmadzadeh,
Jessica Filtjens,
Tania Mitera,
Nele Berghmans,
Philippe E. Van den Steen,
Christin Friedrich,
Georg Gasteiger,
Carine Wouters,
Patrick Matthys”}]]by inmunoadminIn macrophage activation syndrome, a severe cytokine storm syndrome with liver inflammation, liver type 1 innate lymphoid cells (ILC1s) rapidly produce proinflammatory cytokines. However, disease progression results in liver ILC1 apoptosis. In addition, disease development was unaltered in Hobit knockout mice, which lack liver ILC1s, indicating their dispensability for disease. Abstract Macrophage activation syndrome (MAS) … Read more
- Sphingosine‐1‐phosphate receptor type 4 is critically involved in the regulation of peritoneal B‐1 cell trafficking and distribution in vivo. [[{“value”:”Janik Riese,
Annabel Kleinwort,
Maurice Hannemann,
Celine Hähnel,
Stephan Kersting,
Tobias Schulze”}]]by inmunoadminSphingosine-1-phosphate (S1P) is implicated in the regulation of peritoneal B-cell trafficking. Here we show that the effects of S1P on peritoneal B-1 cell migration are mediated by the receptor S1PR4 via direct chemotactic action as well as via upregulation of the expression level of chemokine receptors CXCR4 and CXCR5. Abstract B-1 cells are crucially involved … Read more
- Innate immunity champions: The diverse functions of macrophages. [[{“value”:”Francesca Biscu,
Anissa Zouzaf,
Donatella Cicia,
Clare Pridans,
Gianluca Matteoli”}]]by inmunoadminThis review highlights the pivotal role of macrophages in regulating tissue homeostasis and immune response during disease. Advanced omics technologies reveal their adaptability within tissue-specific niches, influenced by their metabolic states. Understanding these dynamics is crucial for developing targeted therapies that modulate macrophage function, offering innovative strategies for managing chronic inflammatory conditions. Abstract Macrophages are … Read more
- Reduction in mucosal‐associated invariant T cells (MAIT) in APECED patients is associated with elevated serum IFN‐γ concentration. [[{“value”:”Iivo Hetemäki,
Joona Sarkkinen,
Huai Hui Wong,
Nelli Heikkilä,
Saila Laakso,
Simo Miettinen,
Mikko I. Mäyränpää,
Outi Mäkitie,
T Petteri Arstila,
Eliisa Kekäläinen”}]]by inmunoadminWe found a reduced frequency of circulating mucosal-associated invariant T cells (MAITs) in patients with APECED with a clear negative correlation to inflammatory markers CRP, IL-18, and IFN-γ. These findings suggest that the decrease in MAITs is secondary to type 1 immune dysregulation present in the disease. Abstract Mucosal-associated invariant T cells (MAIT) are innate-like … Read more
- γδ T cells in immune‐mediated kidney disease. [[{“value”:”Alex Waterhölter,
Christian F. Krebs,
Ulf Panzer”}]]by inmunoadminThis review summarizes the current knowledge about γδ T cells in immune-mediated kidney diseases. We highlight that γδ T cells are associated with an aggravation of tissue damage through direct pathways and interactions with other leukocyte subsets . Abstract Immune-mediated kidney diseases, including glomerulonephritis (GN), represent a diverse spectrum of disorders characterized by inflammation within … Read more
- Langerhans cells orchestrate apoptosis of DNA‐damaged keratinocytes upon high‐dose UVB skin exposure. [[{“value”:”Daniela Ortner,
Helen Strandt,
Christoph H. Tripp,
Sarah Spoeck,
Athanasios Seretis,
Florian Hornsteiner,
Sophie Dieckmann,
Matthias Schmuth,
Patrizia Stoitzner”}]]by inmunoadminOur study reveals that high-dose UVB exposure stimulates Langerhans cells to release chemokines that recruit neutrophils and induce TNF-α-mediated apoptosis in DNA-damaged keratinocytes. Without Langerhans cells, fewer neutrophils are recruited, leading to an accumulation of DNA-damaged cells and a higher risk of skin cancer due to increased mutations and potential cell transformation. Abstract Ultraviolet (UV) … Read more
- In vitro modulation of T cells in myasthenia gravis by low‐dose IL‐2. [[{“value”:”Merve Çebi,
Arman Çakar,
Hacer Durmuş,
Onur Akan,
Fikret Aysal,
Yeşim Parman,
Güher Saruhan‐Direskeneli”}]]by inmunoadminLow-dose IL-2 balances Tfh, Tph, and Treg cells in myasthenia gravis (MG), by increasing Treg cells and reducing Tfh and Tph cell populations in patients. The results provide in vitro evidence for the use of IL-2 as a potential therapeutic in MG. Abstract Follicular helper (Tfh), peripheral helper (Tph), and regulatory (Treg) T cells are … Read more
- Lipid nanoparticle‐mediated RNA delivery for immune cell modulation. [[{“value”:”Emily H. Kim,
Sridatta V. Teerdhala,
Marshall S. Padilla,
Ryann A. Joseph,
Jacqueline J. Li,
Rebecca M. Haley,
Michael J. Mitchell”}]]by inmunoadminA review of lipid nanoparticle development and application for engineering T cells, NK cells, macrophages, dendritic cells, and stem cells for immunotherapies. Abstract Lipid nanoparticles (LNPs) have emerged as the preeminent nonviral drug delivery vehicles for nucleic acid therapeutics, as exemplified by their usage in the mRNA COVID-19 vaccines. As a safe and highly modular … Read more
- Complement‐targeted therapeutics: Are we there yet, or just getting started?. Daniel Ricklinby inmunoadminAfter a hesitant start, the number of available therapeutics that target the human complement system has surged from one to a dozen drugs in less than 20 years. Meanwhile, the clinical arsenal encompasses treatment modalities directed to several targets for the therapy of a steadily growing spectrum of complement-related disorders. Abstract Therapeutic interventions in the … Read more
- The power of many: Multilevel targeting of representative chemokine and metabolite GPCRs in personalized cancer therapy. [[{“value”:”Donato Inverso,
Carlotta Tacconi,
Serena Ranucci,
Marco De Giovanni”}]]by inmunoadmin(Top). Representative protumorigenic GPCRs can regulate cancer at multiple levels, including immune cell recruitment to TME, angiogenesis, and tumor biology. (Bottom). Multiple and parallel targeting of selected protumorigenic GPCRs might overcome receptor redundancy and impede tumor growth on various fronts, thus increasing the therapeutic potential of GPCR-targeted therapies. Abstract G protein-coupled receptors (GPCRs) are vital … Read more
- Testosterone and estradiol reduce inflammation of human macrophages induced by anti‐SARS‐CoV‐2 IgG. [[{“value”:”Sona Allahverdiyeva,
Chiara E. Geyer,
Jennifer Veth,
Laura M. de Vries,
Steven W. de Taeye,
Marit J. van Gils,
Jeroen den Dunnen,
Hung‐Jen Chen”}]]by inmunoadminOlder and obese individuals are at greater risk of developing severe COVID-19. In this paper, we identified that this may be related to decreased levels of testosterone and estradiol, by showing that these sex hormones reduce the excessive production of proinflammatory cytokines induced by anti-SARS-CoV-2 IgG antibodies. Abstract COVID-19, the disease caused by SARS-CoV-2, particularly … Read more
- ThymoSpheres culture: A model to study human polyclonal unconventional T cells. [[{“value”:”Lore Billiet,
Hanne Jansen,
Melissa Pille,
Lena Boehme,
Guillem Sanchez Sanchez,
Laurenz De Cock,
Glenn Goetgeluk,
Eva Pascal,
Stijn De Munter,
Lucas Deseins,
Joline Ingels,
Thomas Michiels,
Robrecht De Vos,
Amin Zolfaghari,
Niels Vandamme,
Jana Roels,
Tessa Kerre,
Ruslan I. Dmitriev,
Tom Taghon,
David Vermijlen,
Bart Vandekerckhove”}]]by inmunoadminThymoSpheres provide an innovative, user-friendly in vitro model for generating mature, predominantly CD8 single positive T cells. These cells resemble human polyclonal αβ unconventional T cells in transcriptome and TCR repertoire characteristics, making this model valuable for studying the development of this specific T-cell lineage. Abstract In vitro cultures remain crucial for studying the fundamental … Read more