J Leukoc Biol. 2025 Jul 31:qiaf111. doi: 10.1093/jleuko/qiaf111. Online ahead of print.
ABSTRACT
Morphologic studies show increased mast cell activation during T cell-mediated inflammation. Previous research demonstrated that microvesicles from activated T cells (MVT*), but not resting T cells (MVT), stimulate human mast cells via the MAPK pathway, leading to degranulation and cytokine release. This study investigates whether MVT* also promote mast cell migration. MVT and MVT* were isolated from T cell supernatants and mast cell migration was measured using a transwell assay. The molecular mechanisms were analyzed with specific inhibitors. Results showed that MVT* significantly enhanced human mast cells chemotaxis, which dependent on ERK and p38 phosphorylation but not on PI3K. In addition, migration was mediated by the S1P1 receptor rather than S1P2 and by sphingosine kinases 1, indicating a role for S1P1 in MVT*-induced mast cell migration. In summary, MVT* act as chemoattractants, guiding mast cells to inflammatory sites where they become activated, highlighting their importance in T cell-mediated inflammation.
PMID:40743264 | DOI:10.1093/jleuko/qiaf111