Building Tolerance: Employing Genome Engineering Toward Universal Treg Therapy​Julian J Freen-van Heeren on 14 de November de 2025 at 11:00

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J Leukoc Biol. 2025 Nov 14:qiaf164. doi: 10.1093/jleuko/qiaf164. Online ahead of print.

ABSTRACT

Following the 2025 Nobel Prize in Physiology or Medicine for the discovery of regulatory T cells (Tregs), a recent article in Nature Communications reports the creation of allogeneic, genome-edited Tregs engineered to evade immune rejection while retaining suppressive function. Through non-viral CRISPR deletion of B2M and CIITA and insertion of an HLA-E-B2M fusion, the authors produced hypo-immunogenic Tregs that persisted and promoted graft tolerance in humanized mice. In this News and Views, the promise and challenges of such universal “off-the-shelf” Tregs are discussed, emphasizing how genome engineering is reshaping Treg biology into a new era of programmable immune tolerance.

PMID:41234126 | DOI:10.1093/jleuko/qiaf164

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