European Journal of Immunology

The number of exhausted T cells continuously increases in chronic sepsis. Both knockout and “knockdown” of TOX failed to alleviate sepsis-induced T-cell exhaustion. However, deletion of TOX impaired the effector function of T cells, suggesting that TOX is not involved in driving T-cell exhaustion in sepsis. ABSTRACT The immune system undergoes profound dysregulation in sepsis, … Read more

We discovered that the IRE1α−targeting small molecule KIRA6 is a potent inhibitor of the SRC family kinases LYN and FYN. Thereby it prevents mast cell activation by blocking the antigen-triggered activation of the high affinity IgE receptor FcεRI. Thus, KIRA6 is able to abolish mast cell-mediated allergic responses. ABSTRACT Mast cell (MC)-driven allergic diseases are … Read more

CD16+ and CD16− Natural Killer cells and type 3 innate lymphoid cells are found in early human development with distinct distribution and phenotype across fetal tissues. The presence of CD34+RORyt+Eomes± cells selectively in the fetal intestine indicates the presence of tissue-specific progenitors. ABSTRACT The human fetal immune system starts to develop in the first trimester … Read more

Expression of constitutively active STAT6 in IECs of VillinCre_STAT6vt mice leads to enhanced pathology during DSS-induced colitis by regulation of genes involved in various functional pathways associated with epithelial barrier integrity in the colon. ABSTRACT Ulcerative colitis (UC) is an inflammatory bowel disease (IBD) often associated with a Type 2 immune response. Although previous reports … Read more

GLUT1 deficiency prevents glucose uptake in T cells resulting in lower intracellular ATP generation and IFNy production.

IgE-mediated activation of human monocytes results in SHIP-1 activation, IL-10 production, and inhibition of antiviral IP-10. SHIP-1 inhibition by 3AC enhanced IL-10 but suppressed antiviral IP-10. This indicates differential regulatory roles for SHIP-1, which could impact antiviral responses in allergic diseases. ABSTRACT IgE-mediated stimulation of monocytes regulates multiple cellular functions including cellular maturation, cytokine release, … Read more

We here provide detailed protocols for isolating single cell suspension from human nonlymphoid tissues, such as gingiva, lung, intestine, skin, and different tumor tissues as well as tumor-draining lymph nodes. Further, we provide gating strategies for flow cytometric analysis of human DC subpopulations in diverse nonlymphoid tissues. Abstract This article is part of the Dendritic … Read more

Chimeric antigen receptor (CAR) T-cells have proven effective in hematologic malignancies targeting a restricted set of tumor-associated antigens. In this work, we demonstrate that CAR-T cells can be redirected to alternative tumor antigens through a switch protein adapter with antitumor efficacy in preclinical models. ABSTRACT Chimeric antigen receptor-T cell (CAR-T) immunotherapy has shown remarkable results … Read more

Identifying activated T lymphocytes and discerning antigen-specific from bystander T cells is crucial for understanding immune responses. Dual AIM combinations significantly enhance the ability to distinguish antigen-specific from bystander-activated T cells compared with single AIMs. They also have a better positive predictive value and specificity with a modest reduction in sensitivity. ABSTRACT Identifying activated T … Read more

Tumor and immune cells interact through receptor–ligand interactions. B3GNT5 produces nsGSL on tumor cells which is regulated by SPPL3. When SPPL3 is absent, elevated nsGSL levels hinder receptor–ligand binding, leading to reduced immune cell activation. ABSTRACT The development of an effective antitumor response relies on the synergistic actions of various immune cells that recognize tumor … Read more