European Journal of Immunology – Page 42

Med1 controls thymic T‐cell migration into lymph node through enhancer‐based Foxo1‐Klf2 transcription program. [[{“value”:”Ning Yuan, Yanhong Su, Yang Gao, Biao Yang, Tianzhe Zhang, Qianhao Wang, Dan Zhang, Lin Shi, Anjun Jiao, Lei Lei, Lina Sun, Baojun Zhang”}]]

5 de October de 2024 by

(1) Med1 deletion in T cells leads to impaired egress of thymic single-positive cells. (2) Med1 deletion impairs mature T cells’ ability to enter lymph nodes. (3) Med1 promotes Klf2 transcription through facilitating Foxo1 binding to the Klf2 enhancer. Abstract The migration is the key step for thymic T cells to enter circulation and then … Read more

Immune response to cold exposure: Role of γδ T cells and TLR2‐mediated inflammation. [[{“value”:”Daniel Vasek, Peter Holicek, Frantisek Galatik, Anna Kratochvilova, Bianka Porubska, Veronika Somova, Natalie Fikarova, Michaela Hajkova, Martin Prevorovsky, Jitka M Zurmanova, Magdalena Krulova”}]]

5 de October de 2024 by

Our study investigates how short-term and long-term mild cold exposure in rats and regular cold swimmers influences immune response and thermogenesis, highlighting the crucial role of γδ T cells under physiological conditions and TLR2-mediated inflammation. These findings advance our understanding of the complex interactions between thermogenesis, neural signaling, and immune regulation. Abstract The mammalian body … Read more

Tridecylcyclohexane in incomplete Freund’s adjuvant is a critical component in inducing experimental autoimmune diseases. [[{“value”:”Norifumi Iijima, Tomoya Hayashi, Masaaki Niino, Yoichi Miyamoto, Masahiro Oka, Ken J Ishii”}]]

5 de October de 2024 by

Autoantigen and heat-killed Mycobacterium tuberculosis emulsified in incomplete Freund’s adjuvant (IFA) are capable of inducing numerous autoimmune diseases in animal models. However, the role of IFA components in triggering these diseases remains unknown. In this study, the emulsification with tridecylcyclohexane was critical for the pathogenesis of autoimmune diseases in mice. Abstract Incomplete Freund’s adjuvant (IFA) … Read more

RAG1/2 induces double‐stranded DNA breaks at non‐Ig loci in the proximity of single sequence repeats in developing B cells. [[{“value”:”Katarina Ochodnicka‐Mackovicova, Michal Mokry, Martin Haagmans, Ted E. Bradley, Carel J.M. van Noesel, Jeroen E.J. Guikema”}]]

5 de October de 2024 by

RAG1/2 endonuclease induces off-target DNA breaks in developing B cells, highlighting its genotoxic potential beyond the immunoglobulin loci. This genome-wide study in mouse pre-B cells identifies RAG1/2-dependent DSBs and uncovers their predominant association with GA and CA dinucleotide repeats and GC-rich motifs, providing new insights into RAG1/2-targeting. Abstract In developing B cells, V(D)J gene recombination … Read more

Regulation of IFN‐γ production by ZFP36L2 in T cells is time‐dependent. [[{“value”:”Nordin D. Zandhuis, Aurélie Guislain, Abeera Popalzij, Sander Engels, Branka Popović, Martin Turner, Monika C. Wolkers”}]]

5 de October de 2024 by

We uncovered a novel time-dependent role for the RNA-binding protein ZFP36L2 in regulating IFN-γ production. ZFP36L2 was redundant in regulating cytokine production during early T-cell activation. However, upon continued T-cell activation, ZFP36L2 dampens the production of IFN-γ by binding to AU-rich elements in the 3ʹUTR and promoting mRNA degradation. Abstract CD8+ T cells kill target … Read more

Functional Fc receptors are crucial in antibody‐mediated protection against cytomegalovirus. [[{“value”:”Anna Bootz, Nina Reuter, Falk Nimmerjahn, William J. Britt, Michael Mach, Thomas H. Winkler”}]]

5 de October de 2024 by

The mechanisms of protection of therapeutically applied antibodies in cytomegalovirus infection are so far unknown. Here it is shown in an in vivo mouse model that Fc-receptors are essential for protection from a lethal infection in immunodeficient hosts. Whereas NK cells have a limited role, monocytes are involved in protection. Created with BioRender.com. Abstract Human … Read more

Site‐specific serology unveils cross‐reactive monoclonal antibodies targeting influenza A hemagglutinin epitopes. [[{“value”:”Philipp C. G. Paparoditis, Alexander Fruehwirth, Kajetana Bevc, Jun Siong Low, Josipa Jerak, Laura Terzaghi, Mathilde Foglierini, Blanca Fernandez, David Jarrossay, Davide Corti, Federica Sallusto, Antonio Lanzavecchia, Antonino Cassotta”}]]

5 de October de 2024 by

The authors developed a novel blocking of binding (BoB)-ELISA to accurately estimate the titers of serum antibodies targeting distinct influenza hemagglutinin (HA) epitopes. Leveraging BoB-ELISA, the authors found that serum antibodies targeting the HA head epitopes were immunodominant, whereas antibodies against the stem epitope, although subdominant, were highly prevalent in healthy donors. Importantly, BoB-ELISA allowed … Read more

Highly sensitive reporter cell line for detection of interferon types I–III and their neutralization by antibodies. [[{“value”:”Kevin Groen, Roger Kuratli, Lauren Sar, Andri Vasou, Michael Huber, David J. Hughes, Benjamin G. Hale”}]]

5 de October de 2024 by

Interferons (IFNs) are a critical component of innate immune defenses and limit viral disease severity. To advance studies on IFNs and their neutralization by pathogenic autoantibodies, we generated a Renilla luciferase-based reporter cell line capable of detecting the activities of IFN-Is, IFN-II, and IFN-IIIs. The reporter cell line exhibits a 125- to 2000-fold higher sensitivity … Read more

A20 intrinsically influences human effector T‐cell survival and function by regulating both NF‐κB and JNK signaling. [[{“value”:”Gina Dabbah‐Krancher, Allison Ruchinskas, Melissa A. Kallarakal, Katherine P. Lee, Bradly M. Bauman, Benjamin Epstein, Hongli Yin, Daniel Krappmann, Brian C. Schaefer, Andrew L. Snow”}]]

3 de October de 2024 by

The ubiquitin-editing enzyme A20 regulates innate and adaptive immune receptor signaling, but its intrinsic function in human T cells is poorly understood. We show that A20 deletion enhances cytokine secretion and alters apoptosis sensitivity in primary human T cells by modulating NF-kB and JNK/AP-1 signaling downstream of the CARD11-BCL10-MALT1 complex. Abstract A20 is a dual-function … Read more

Chronic stimulation desensitizes β2‐adrenergic receptor responses in natural killer cells. [[{“value”:”Martin Jürgens, Maren Claus, Sabine Wingert, Jens Alexander Niemann, Lea Katharina Picard, Elisabeth Hennes, Ina Haasler, Birte Hellwig, Nina Overbeck, Jörg Reinders, Jörg Rahnenführer, Michaela Schedel, Silvia Capellino, Carsten Watzl”}]]

1 de October de 2024 by

β2-adrenergic receptor (β2AR) stimulation of NK cells inhibits inside-out signaling and LFA-1 activation, explaining the mobilization of NK cells upon acute stress. Repeated or sustained β2AR stimulation desensitizes β2AR via G-protein switch. Local administration of long-acting β2-agonists does not interfere with systemic NK cell functions in asthma patients. Abstract Adrenergic receptors (ARs) are preferentially expressed … Read more