J Immunol. 2025 Nov 11:vkaf301. doi: 10.1093/jimmun/vkaf301. Online ahead of print. ABSTRACT Antigen-specific responses to complex antigens encompass a range of cell states and reactivities to an array of epitopes, reflective of the heterogeneity in immune responses. Single-cell sequencing has created new opportunities when combined with flow cytometry for profiling of immune repertoire and cell … Read more

J Immunol. 2025 Nov 11:vkaf273. doi: 10.1093/jimmun/vkaf273. Online ahead of print. ABSTRACT Dendritic cells (DCs) and B lymphocytes produce major histocompatibility complex class II molecules (MHCIIs) in large amounts to maximize the display of peptides and fulfill their antigen-presentation functions. The surface expression of MHCIIs in these cells is regulated via the ubiquitination of a … Read more

J Immunol. 2025 Nov 11:vkaf306. doi: 10.1093/jimmun/vkaf306. Online ahead of print. ABSTRACT The sympathetic nervous system modulates immune responses through the release of norepinephrine (NE), yet the dynamics of this signaling differ across lymphoid organs. In this study, we investigated how NE release and α2-adrenergic receptor (α2AR) modulation influence neuroimmune interactions in the spleen and … Read more

J Immunol. 2025 Nov 11:vkaf309. doi: 10.1093/jimmun/vkaf309. Online ahead of print. ABSTRACT STAT3 is pivotal for governing myeloid responses to inflammatory stimuli to prevent hyperinflammation in vivo, yet whether STAT3 mediates pathogen control and clearance by myeloid cells remains unclear. In this study, we identified significant enrichment of IFN-stimulated transcriptional pathways in Stat3-deficient bone marrow-derived … Read more

J Immunol. 2025 Nov 10:vkaf292. doi: 10.1093/jimmun/vkaf292. Online ahead of print. ABSTRACT Artificial intelligence (AI) and machine learning (ML) are transforming biotechnology and playing a key role in bioeconomy. One of the most important measurement capabilities at the forefront of biotechnology innovations is flow cytometry (FCM), a high-throughput, single-cell analysis platform technology. However, the quality … Read more

J Immunol. 2025 Nov 10:vkaf300. doi: 10.1093/jimmun/vkaf300. Online ahead of print. ABSTRACT Mucosal immunoglobulin A (IgA) promotes the survival of commensal bacteria while it inhibits the invasion by pathogens. Bacterial coating may be mediated by antigen-specific IgA recognition, polyreactivity, and/or by the IgA-associated glycans. We compared human polyclonal secretory SIgA both in vitro and in … Read more

J Immunol. 2025 Nov 9:vkaf260. doi: 10.1093/jimmun/vkaf260. Online ahead of print. ABSTRACT Mucosal-associated invariant T (MAIT) cells are highly conserved innate-like T cells in mammals recognized for their high baseline frequency in human blood and cytotoxic effector functions during infectious diseases, autoimmunity, and cancer. While the majority of these cells in humans express a conserved … Read more

J Immunol. 2025 Nov 9:vkaf280. doi: 10.1093/jimmun/vkaf280. Online ahead of print. ABSTRACT Cigarette smoke is the primary cause of chronic obstructive pulmonary disease (COPD), an incurable condition characterized by irreversible airflow obstruction and alveolar destruction driven by chronic inflammation of the lungs and airways. The inflammatory response caused by cigarette smoke is typified by the … Read more

J Immunol. 2025 Nov 6:vkaf218. doi: 10.1093/jimmun/vkaf218. Online ahead of print. ABSTRACT Increasing evidence indicates that invertebrates have the immune adaptive abilities, which is commonly known as “immune priming”. However, the mechanism of immune priming is believed to be rather complicated, and it has not been fully elucidated. In the present study, in the oysters … Read more

J Immunol. 2025 Nov 7:vkaf262. doi: 10.1093/jimmun/vkaf262. Online ahead of print. ABSTRACT Human whole blood (WB) immunophenotyping may represent the in vivo immunological state with better fidelity than artificially isolated peripheral blood mononuclear cells (PBMCs). We used a deep phenomics modeling approach to elucidate the quantitative differences in major immune cell lineages in WB and … Read more