Evaluation of the viability and functionality of human peripheral blood mononuclear cells cryopreserved up to 2 years in animal-protein-free freezing media compared to the FBS-supplemented reference medium
BackgroundCryopreservation of peripheral blood mononuclear cells (PBMCs) is crucial for consistent analysis in immunological studies and clinical trials. Traditional freezing media often contain fetal bovine serum (FBS), which raises ethical concerns a…