This 23-color mouse immunophenotyping panel was designed and developed by the Virginia Commonwealth University’s (VCU) flow cytometry shared resource (FCSR) to easily bring new use to our high-parameter spectral cytometers. Our method is broadly applic…
Mucosal infections are a major global health concern in humans and animals. Neutralization assays are considered the gold standard for evaluating functional antibodies but are typically limited to serum analysis. This often excludes mucosal sample type…
IntroductionQuantitative investigation of how the spatial organization of cells within the tumor microenvironment associates with disease progression, patient outcomes, and that cell’s phenotypic state remains a key challenge in cancer biology. High-di…
Complement opsonin C3b is at the center of the complement cascade. All complement initiation pathways converge when convertases activate C3, converting it into the anaphylatoxin C3a and the opsonin C3b, which can attach to surfaces via its freshly expo…
Hypereosinophilic syndrome (HES) is a heterogeneous group of disorders characterised by persistent hypereosinophilia associated with organ damage. Due to its rarity and heterogeneity in its clinical presentation, HES remains underdiagnosed or misdiagno…
BackgroundA novel fully automated chemiluminescent immunoassay (CLIA) has been developed to quantify autoantibodies specific for desmogleins (Dsg1, Dsg3), BP180, and BP230, key target antigens in pemphigus and bullous pemphigoid. This study aims to eva…
Dengue is caused by the four serotypes of dengue virus (DENV-1-4) and poses a significant global public health challenge, with an estimated 100–400 million infections annually. Severe dengue manifestations, such as Dengue Hemorrhagic Fever (DHF) and De…
Human leukocyte antigen (HLA) molecules are pivotal in guiding human adaptive immune responses through their presentation of peptide ligands, collectively known as the immunopeptidome. This process is central to the development of cancer immunotherapie…
There are ten leukocyte immunoglobulin (Ig)-like receptor (LILR) genes, i.e., five genes encoding activating receptors (LILRA1, LILRA2, LILRA4, LILRA5, and LILRA6) characterized by their truncated cytoplasmic tails, and five genes encoding inhibitory r…
Chimeric antigen receptor (CAR)-modified cell therapy products approved for clinical treatment of hematological malignancies have hitherto been based on T cells. NK cells represent a promising immune cell type that can be considered for CAR engineering…
