Genetic engineering of primary hematopoietic cells is essential for mechanistic immunology studies, however the development of cell-based therapies yet remains constrained by two major factors: the fragility of many primary lineages and challenges of v…
Accurate identification and spatial enumeration of B lineage cells in formalin-fixed, paraffin-embedded (FFPE) lupus nephritis (LN) kidney tissue is critical for understanding disease pathogenesis and CD20-directed therapeutic responses. We developed a…
Tuberculomas are the conglomeration of tuberculous granulomas into structurally organized three-dimensional (3D) masses that result from Mycobacterium tuberculosis infection and represent one of the more severe morphological forms of tuberculosis (TB)….
NETosis is a distinct form of neutrophil cell death involved in innate immunity and characterized by the release of DNA, that when dysregulated contributes to tissue damage and target-organ injury. As our understanding of the mechanisms governing this …
This 23-color mouse immunophenotyping panel was designed and developed by the Virginia Commonwealth University’s (VCU) flow cytometry shared resource (FCSR) to easily bring new use to our high-parameter spectral cytometers. Our method is broadly applic…
Mucosal infections are a major global health concern in humans and animals. Neutralization assays are considered the gold standard for evaluating functional antibodies but are typically limited to serum analysis. This often excludes mucosal sample type…
IntroductionQuantitative investigation of how the spatial organization of cells within the tumor microenvironment associates with disease progression, patient outcomes, and that cell’s phenotypic state remains a key challenge in cancer biology. High-di…
Complement opsonin C3b is at the center of the complement cascade. All complement initiation pathways converge when convertases activate C3, converting it into the anaphylatoxin C3a and the opsonin C3b, which can attach to surfaces via its freshly expo…
Hypereosinophilic syndrome (HES) is a heterogeneous group of disorders characterised by persistent hypereosinophilia associated with organ damage. Due to its rarity and heterogeneity in its clinical presentation, HES remains underdiagnosed or misdiagno…
BackgroundA novel fully automated chemiluminescent immunoassay (CLIA) has been developed to quantify autoantibodies specific for desmogleins (Dsg1, Dsg3), BP180, and BP230, key target antigens in pemphigus and bullous pemphigoid. This study aims to eva…
