The C-terminal of TSP-1 senses cell contact, which triggers N-terminal cleavage by urokinase, adhesion, and cytoplasmic spreading in T cells and platelets. In contrast, full-length TSP-1 induces the development of polar cell shape and migration in T cells 2D and 3D; polar cell shape 2D, independent of its receptor LRP1, and polar cell shape 3D … Read more

This review summarizes emerging spatially resolved multi-omics approaches revealing organized cell–cell interactions in skeletal muscle and brain inflammation. These tools uncover radiating molecular programs and niche-specific immunopathology that shape cellular reactivity and vulnerability. ABSTRACT Spatial omics technologies enable high-resolution mapping of transcriptomic, proteomic, and metabolic profiles within intact tissues, revealing how immune, stromal, and parenchymal … Read more

CCR7-mediated chemokine signaling regulates the medullary migration of thymocytes to establish self-tolerance. By comparing the thymic phenotype of Ccl21b-KO mice with that of Ccl21a-KO mice, we show that Ccl21a plays a predominant role compared with Ccl21b among CCR7 ligands in the thymus. ABSTRACT Self-tolerance in T cells is a vital self-defense strategy for mammals to … Read more

HLDA Workshops were established 40 years ago to develop a standardized CD molecule nomenclature for leukocyte cell-surface molecules by comparing monoclonal antibodies (mAbs) produced by different laboratories and companies. The HLDA11 Workshop focused on validating G-protein-coupled receptors (GPCRs) targeting mAbs and assessing cell-type-specific expression patterns in leukocytes. ABSTRACT Monoclonal antibodies (mAbs) targeting cell-surface molecules are … Read more

The RNA-binding ROQUIN paralogs are critical immune regulators. We demonstrate that mast cell-specific deficiency for ROQUIN-1 and ROQUIN-2 in mice leads to loss of mast cells in serous cavities. ROQUIN-1 and 2 ablation does not affect mast cell degranulation or proinflammatory cytokine secretion, although they regulate many mRNAs directly or indirectly. ABSTRACT Mast cells are … Read more

SARS-CoV-2 vaccination in rheumatoid arthritis leads to the development of CD4+ and CD8+ spike-specific T cell memory, regardless of immunosuppressive therapies, and the number of vaccine injections. CD4−CD8− double-negative (DN) T cells responded to SARS-CoV-2 peptide epitopes and differentiated into CD8+ cytotoxic T cells after stimulation, suggesting a role in exacerbating the inflammation in RA … Read more

Antibody feedback dynamically shapes the selective landscape for GC B-cells, acting as a driving force of Darwinian-like affinity maturation of B-cell receptors (A). It also balances epitope usage, leading to equal competition among B cell clones specific for different epitopes and allowing the emergence of clones specific for new epitopes (B). ABSTRACT Antibody feedback in … Read more

Early B cell activation requires both FcµR and IgM-mediated complement signaling, which together drive initial clonal expansion, class-switch recombination, germinal center (GC) entry, and plasma cell differentiation. During the GC reaction, IgM BCR-mediated complement activation, but not FcµR, supports GC B cell survival, proliferation, and affinity maturation. ABSTRACT Secretory IgM plays a pivotal role in … Read more

Murine allergy model outcomes differ by sensitization route, allergen type, and mouse strain, influencing mechanisms such as IgE and mast cell involvement and the therapeutic relevance of each model. Understanding how experimental parameters shape outcomes is essential for selecting appropriate models and effectively translating findings to clinical applications. ABSTRACT Immunoglobulin E (IgE)–mediated immediate hypersensitivity reactions … Read more

Staphylococcal enterotoxin A (SEA) alters monocyte differentiation and function, while preserving T cell stimulatory capacity. SEA-primed macrophages downregulate antigen-presenting markers yet drive heightened T-cell proliferation and IFN-γ secretion. These findings reveal mechanisms of SEA-mediated immune modulation and superantigen-driven inflammation. ABSTRACT Staphylococcal enterotoxins (SE) crosslink the MHC-II on antigen-presenting cells (APC) with the T-cell receptor, inducing … Read more