This study demonstrates that CD27−CD21lo B cells harbor a relatively low frequency of somatic mutations in their BCR compared with CD27+CD21lo B cells and early plasmablasts. Clonal relationships were present among all three subsets in both health and disease, indicating that CD27−CD21lo B cells actively contribute to immune responses. ABSTRACT B cells with low or … Read more

Human CD8αβ was produced as an Fc-fusion via knob-in-hole mediated heterodimerization and bound class I pHLA and MR1 with similar affinity to human CD8αα, but did not bind CD1a, b, c, or d. An inverse relationship between CD8:pHLA affinity and TCR:pHLA was observed for several alleles. ABSTRACT The CD8 co-receptor exists as both an αα … Read more

We engineered the IgG-degrading enzyme IdeS as a Fc-fusion protein. IdeS-Fc exhibits similar hydrolysing efficacy to its native counterpart, but has an increased half-life, translating into prolonged therapeutic degradation of IgG. ABSTRACT Imlifidase (IdeS) is a bacterial protease that hydrolyzes human IgG in their hinge region, decreasing their half-life and abrogating their Fc-mediated properties. It … Read more

Following isolation and expansion, the function of Tregs can be enhanced through mRNA electroporation, resulting in protein overexpression. Our findings reveal that protein secretion post-electroporation is dependent on the activation state of the Tregs. Specifically, only activated Tregs were capable of successfully secreting BDNF after BDNF-mRNA electroporation. ABSTRACT Genetic engineering of regulatory T cells (Tregs) … Read more

BAFF blockade suppresses B cell MALT formation in H. felis-infected Nlrc5mø-KO mice. The upregulation of protein-coding BAFF transcripts in NLRC5-deficient macrophages suggests a novel NLRC5–BAFF signalling axis. ABSTRACT Helicobacter infection is a key cause of gastric B cell mucosa–associated lymphoid tissue (MALT) lymphoma. This study examined the role of B cell–activating factor (BAFF), a major … Read more

The number of exhausted T cells continuously increases in chronic sepsis. Both knockout and “knockdown” of TOX failed to alleviate sepsis-induced T-cell exhaustion. However, deletion of TOX impaired the effector function of T cells, suggesting that TOX is not involved in driving T-cell exhaustion in sepsis. ABSTRACT The immune system undergoes profound dysregulation in sepsis, … Read more

We discovered that the IRE1α−targeting small molecule KIRA6 is a potent inhibitor of the SRC family kinases LYN and FYN. Thereby it prevents mast cell activation by blocking the antigen-triggered activation of the high affinity IgE receptor FcεRI. Thus, KIRA6 is able to abolish mast cell-mediated allergic responses. ABSTRACT Mast cell (MC)-driven allergic diseases are … Read more

CD16+ and CD16− Natural Killer cells and type 3 innate lymphoid cells are found in early human development with distinct distribution and phenotype across fetal tissues. The presence of CD34+RORyt+Eomes± cells selectively in the fetal intestine indicates the presence of tissue-specific progenitors. ABSTRACT The human fetal immune system starts to develop in the first trimester … Read more

Expression of constitutively active STAT6 in IECs of VillinCre_STAT6vt mice leads to enhanced pathology during DSS-induced colitis by regulation of genes involved in various functional pathways associated with epithelial barrier integrity in the colon. ABSTRACT Ulcerative colitis (UC) is an inflammatory bowel disease (IBD) often associated with a Type 2 immune response. Although previous reports … Read more

GLUT1 deficiency prevents glucose uptake in T cells resulting in lower intracellular ATP generation and IFNy production.