European Journal of Immunology

Managing Autofluorescence in Spectral Flow Cytometry for Macrophage Identification in the Liver. [[{“value”:”Sabine Daemen, Tuneille I. Adelaar, Panos Barlampas, Xiaodi Zhang, Casper G. Schalkwijk, Kristiaan Wouters”}]]

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Autofluorescence in liver tissue is heterogeneous and dynamically altered during metabolic liver disease, complicating spectral flow cytometry analysis. We provide a structured framework for identifying and handling condition-dependent autofluorescence, demonstrating that inadequate autofluorescence correction biases macrophage quantification and subset resolution in complex liver samples. ABSTRACT Autofluorescence (AF) in biological tissues arises from the natural emission … Read more

Characterization of Harderian Gland‐Derived Plasma Cells in Chicken. [[{“value”:”D. von La Roche, I. Naumann, E. Szőcs, V. Halasy, M. Kohn, B. Kaspers, N. Nagy, S. Härtle”}]]

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An isolation protocol for plasma cells (PC) from the chicken Harderian gland (HG) was established, and analyses of PCs revealed a FSChigh/CD45med/sIgpos/CD57pos/CXCR4high/TACIlow phenotype. Presence of CXCL12 and BAFF around HG PCs suggests a contribution of CXCL12–CXCR4 and BAFF–TACI to PC localization and survival. ABSTRACT Due to fundamental differences in B cell development between birds and … Read more

Stable β2‐Microglobulin–HLA Class I Association Reshapes the Antigenic Landscape and TCR Recognition of Cancer‐Associated Epitopes. [[{“value”:”Ana Barajas Molina, Yehor Horokhovskyi, Nicole Acuti, Yee Kien Chong, Meghna Phanichkrivalkosil, Sophie Siddons, Hassnae Afrache, Megan Maloney, Wai Tuck Soh, Tom Hofland, Callum Hall, Henning Urlaub, George Kassiotis, Juliane Liepe, Michele Mishto”}]]

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We investigated differences in peptide repertoire when β2-microglobulin is fused to the HLA-I heavy chain. We observed: (1) that immunopeptidomes changed in this condition, (2) an overall increased median IC50 of the entire peptide repertoire, and (3) a reduced presentation of cancer-specific epitopes with a strong predicted binding affinity for HLA-I. ABSTRACT β2-Microglobulin (β2m) is … Read more

STING Activation in Dendritic Cells Enhances Antigen Presentation by Live Tumor Cells. [[{“value”:”Adriana Loverre, Bakhos Jneid, Magali Genest, Maries van den Broek, Nicolas Manel”}]]

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Our study reveals that STING activation in dendritic cells using cGAMP-VLP enhances CD8+ T cell recognition of live tumor cells by inducing type I interferon-dependent upregulation of MHC-I on tumor cells in vitro. In vivo, cGAMP-VLP boosts antitumor immunity even in cGAS-deficient tumors, thereby improving the efficacy of immune checkpoint blockade. These findings uncover a … Read more

High‐dimensional Spatial Immune Profiling Highlights Microglia‐Like Cells in Human Dorsal Root Ganglia. [[{“value”:”Marius Schwabenland, Busranur Oeztuerk, Thomas Blank, Juergen Beck, Bertram Bengsch, Marco Prinz”}]]

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High-dimensional imaging mass cytometry reveals diverse macrophage populations in human dorsal root ganglia (DRG). Microglia-like cells, characterized by markers such as P2RY12, SLC2A5, and TMEM119, localize near neuronal structures, whereas other myeloid subsets are found in interstitial regions. Spatial analysis reveals distinct localization patterns within the DRG. ABSTRACT The human dorsal root ganglia (DRG) are … Read more

Sialylation in Colorectal Cancer Rewires Antitumor Immunity at the Peritoneal Metastatic Site. [[{“value”:”Irene van der Haar Àvila, Kristiaan Lenos, Victor Lorrain, Eleonora Nardini, Ernesto Rodríguez, Juan J. García‐Vallejo, Yvette van Kooyk, Joep Grootjans, Sandra J. van Vliet”}]]

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This study shows that loss of sialylation in CT26 cells reduces peritoneal tumor burden, correlating with a lower peritoneal cancer index score. These tumors exhibited increased immune infiltration with prominent alterations in the myeloid compartment and immune cell correlations unique to desialylated tumors, indicating profound reshaping of antitumor immunity. ABSTRACT Colorectal cancer (CRC) patients are … Read more

The RNA‐ and DNA‐Binding Protein Y‐Box Binding Protein 1 (YB‐1) Regulates iNKT Cell Development. [[{“value”:”Silvia Schulze, Laura Knop, Nouria Jantz‐Naeem, Vladyslava Dovhan, Stephan Fricke, Sascha Kahlfuß, Thomas Schüler, Ursula Bommhardt”}]]

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YB-1 is essential for invariant NKT cell development, integrating TCR, co-stimulatory, and IL-15 signaling to control maturation, subset differentiation, and survival. Its loss at the double-positive thymocyte stage causes ∼80% iNKT cell depletion, maturation arrest, impaired cytokine production, and increased apoptosis. ABSTRACT Y-box binding protein 1 (YB-1) is a multifunctional RNA- and DNA-binding protein with … Read more

A Novel PTPN2 Isoform Differentially Regulates Immune Response. [[{“value”:”Xiaoguang Li, Fedik Rahimov, Rui Wang, Lei Gao, Renze Ma, Tiankai Han, Yingtao Bi, Freidrich Cruz, Jing Wang, Shaun Grosskurth, Elizabeth Asque, Jesus Paez‐Cortez, Marc C. Levesque, Nancy E. Crosbie, Timothy Radstake, Amlan Biswas, Kanstantsin Katlinski”}]]

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The A allele of the intronic SNP rs80262450 in the PTPN2 gene favors the expression of the splicing isoform PTPN2.4. This cytoplasmic isoform enhances TNF-α production in myeloid cells, thereby contributing to intestinal inflammation. These findings identify PTPN2.4 as a key pathogenic regulator and potential therapeutic target for inflammatory bowel disease. ABSTRACT Genome-wide association studies … Read more

Hypoxia Independently Induces AID Expression in CH12 B Cells. [[{“value”:”Vincent Heyer, Sandy Haidar Ahmad, Mira Haddad, Inès Argentieri, Coraline Gessier, Bernardo Reina‐San‐Martin”}]]

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Hypoxia activates the HIF complex (HIF-1α, HIF-1β, HIF-2α) to induce AID expression in B cells independently of cytokine signaling. However, hypoxia alone is insufficient to trigger class switch recombination (CSR), which additionally requires cytokine-driven chromatin accessibility, highlighting a mechanistic uncoupling between AID induction and functional antibody diversification. ABSTRACT Hypoxia has been shown to shape the … Read more

Sustained Effector Functions and Memory Accumulation of αβ T Cells in Children With Congenital Heart Disease. [[{“value”:”Yusuf Eshimutu Abu, Christoph Kammeyer, Tao Yang, Rodrigo Gutierrez Jauregui, Alexa Cramer, Claudia Junge, Svea Kleiner, Anika Janssen, Adan Chari Jirmo, Alexander Horke, Reinhold Förster, Philipp Beerbaum, Martin Boehne, Sarina Ravens”}]]

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This study showed dysregulated αβ T cell homeostasis with ongoing T cell activation and low-grade inflammation in 5-12-year-old CHD children who underwent early-life cardiac surgery with thymectomy, characterized by reduced naïve T cells and increased memory/effector and senescent-like T cells. There was also higher expression of inhibitory receptors on effector T cells and elevated suppressive … Read more

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