J Leukoc Biol. 2024 Oct 3:qiae215. doi: 10.1093/jleuko/qiae215. Online ahead of print. ABSTRACT Approximately one million cases of sepsis in the U.S.A. occur annually. The early phase of sepsis features dramatic changes in host metabolism and inflammation. While examining the effects of metabolic pathways on inflammation, we discovered that the highly reactive glycolytic metabolite, methylglyoxal … Read more

J Leukoc Biol. 2024 Oct 1:qiae198. doi: 10.1093/jleuko/qiae198. Online ahead of print. ABSTRACT Treatment with the toll-like receptor (TLR) 4 agonist monophosphoryl lipid A (MPLA) conditions innate immunocytes to respond robustly to subsequent infection, a phenotype termed innate immune memory. Our published studies show that metabolic reprogramming of macrophages is a prominent feature of the … Read more

J Leukoc Biol. 2024 Sep 28:qiae211. doi: 10.1093/jleuko/qiae211. Online ahead of print. ABSTRACT Septic shock is associated with over 40% mortality. The immune response in septic shock is tightly regulated by cellular metabolism and transitions from early hyper-inflammation to later hypo-inflammation. Patients are susceptible to secondary infections during hypo-inflammation. The magnitude of the metabolic dysregulation … Read more

J Leukoc Biol. 2024 Sep 27:qiae212. doi: 10.1093/jleuko/qiae212. Online ahead of print. ABSTRACT Viral RNA and miRNAs released by immune cells contribute to inflammation in COVID-19 patients. Here, we investigated the role of SARS-CoV2 RNA and host miRNAs carried within extracellular vesicles (EVs) in modulating inflammation. EVs were classified as positive or negative depending on … Read more

J Leukoc Biol. 2024 Sep 25:qiae209. doi: 10.1093/jleuko/qiae209. Online ahead of print. ABSTRACT Neutrophils are highly abundant in the gingival tissues where they play an essential role in immune homeostasis by preventing microbial invasion. Here, we show that the oral periodontal pathogen Porphyromonas gingivalis utilizes its cysteine proteases (gingipains) to disengage phagosomal antimicrobial capacity. Arginine … Read more

J Leukoc Biol. 2024 Sep 25:qiae208. doi: 10.1093/jleuko/qiae208. Online ahead of print. ABSTRACT A majority of genetically modified mice have been produced using 129 strain-derived embryonic stem cells (ESCs). Despite ample backcrosses with other strains, these may retain characteristic for 129 passenger mutations leading to confounding phenotypes unrelated to targeted genes. Here we show that … Read more

J Leukoc Biol. 2024 Sep 23:qiae207. doi: 10.1093/jleuko/qiae207. Online ahead of print. ABSTRACT In humans and in mice, maternal allergy predisposes offspring to development of allergy. In murine models, increased levels of maternal β-glucosylceramides are both necessary and sufficient for the development of allergic predisposition in offspring. Furthermore, increased numbers of CD11b+ dendritic cell subsets … Read more

J Leukoc Biol. 2024 Sep 23:qiae206. doi: 10.1093/jleuko/qiae206. Online ahead of print. ABSTRACT Newborns are at high risk to develop sepsis. This is linked to innate immune responses at birth which are not completely adapted to postnatal life. Neutrophils are key players of innate immunity and exhibit a marked ontogenetic regulation of their functionality. Here, … Read more

J Leukoc Biol. 2024 Sep 23:qiae204. doi: 10.1093/jleuko/qiae204. Online ahead of print. ABSTRACT We report on a pilot study exploring whether blood immune signatures can reveal early specific indicator profiles for patients meeting sepsis criteria upon hospital admission. We analysed samples of sepsis-suspected patients (N=20) and age-spanning healthy controls (N=12), using flow cytometry-based assays. We … Read more

J Leukoc Biol. 2024 Sep 20:qiae205. doi: 10.1093/jleuko/qiae205. Online ahead of print. ABSTRACT The cytokine interferon-gamma (IFNγ) plays a multifaceted role in intestinal immune responses ranging from anti- to pro-inflammatory depending on the setting. Here, using a 3D co-culture system based on human intestinal epithelial organoids, we explore the capacity of IFNγ-exposure to reprogram intestinal … Read more