Resolving the renal microenvironment: a 5-plex immunofluorescence workflow to quantify B-lineage cells in FFPE lupus nephritis biopsies
Accurate identification and spatial enumeration of B lineage cells in formalin-fixed, paraffin-embedded (FFPE) lupus nephritis (LN) kidney tissue is critical for understanding disease pathogenesis and CD20-directed therapeutic responses. We developed a…