Is there an alternative to the indirect immunofluorescence ANA HEp-2 assay for the diagnosis of connective tissue diseases?
BackgroundAnti-nuclear antibodies (ANA) are serological hallmarks in the diagnosis of ANA-associated rheumatic diseases (AARD). For many years, indirect immunofluorescence (IIF) on HEp-2 cell substrate has been regarded as the gold standard method for …