European Journal of Immunology

This study established a 21-day in vitro culture system for human long-lived plasma cells (LLPCs), demonstrating their survival and antibody secretion depend on stromal cell contact. PI3K and NF-kappaB pathways were crucial for LLPC survival, aligning with findings in mice. This model advances the understanding of human LLPC biology. ABSTRACT Contrary to short-lived plasma cells, … Read more

Allergen-specific antibodies can be detected in a large proportion of individuals. Still, only a fraction suffer from clinical signs of allergic disease, and of these, some, but not all, will develop more severe forms of the disease over time or respond to allergen-specific immunotherapy. Here, we review the concept of nonpathogenic versus pathogenic antibody responses … Read more

Sun et al. demonstrate that deficiency of macrophage-intrinsic Ubc13 increases Akt activation and PD-L1 expression, which triggers macrophage protumoral polarization and lung tumor progression. Ubc13-depletion in macrophage also increases infiltration of myeloid-derived suppressor cells and decreases proliferative and effector function of CD8+ T lymphocytes in the tumor microenvironment. ABSTRACT Tumor cell-intrinsic ubiquitin-conjugating enzyme Ubc13 promotes tumorigenesis, … Read more

ABSTRACT The journey of early-career researchers (ECRs) in immunology is marked by many challenges but also exciting prospects. Here we bring a snapshot of the recent ECR workshop organized by the Young European Federation of Immunology Societies (yEFIS) during the 7th European Congress of Immunology (ECI), which took place in Dublin Ireland from September 1 … Read more

This study demonstrates that CD27−CD21lo B cells harbor a relatively low frequency of somatic mutations in their BCR compared with CD27+CD21lo B cells and early plasmablasts. Clonal relationships were present among all three subsets in both health and disease, indicating that CD27−CD21lo B cells actively contribute to immune responses. ABSTRACT B cells with low or … Read more

Human CD8αβ was produced as an Fc-fusion via knob-in-hole mediated heterodimerization and bound class I pHLA and MR1 with similar affinity to human CD8αα, but did not bind CD1a, b, c, or d. An inverse relationship between CD8:pHLA affinity and TCR:pHLA was observed for several alleles. ABSTRACT The CD8 co-receptor exists as both an αα … Read more

We engineered the IgG-degrading enzyme IdeS as a Fc-fusion protein. IdeS-Fc exhibits similar hydrolysing efficacy to its native counterpart, but has an increased half-life, translating into prolonged therapeutic degradation of IgG. ABSTRACT Imlifidase (IdeS) is a bacterial protease that hydrolyzes human IgG in their hinge region, decreasing their half-life and abrogating their Fc-mediated properties. It … Read more

Following isolation and expansion, the function of Tregs can be enhanced through mRNA electroporation, resulting in protein overexpression. Our findings reveal that protein secretion post-electroporation is dependent on the activation state of the Tregs. Specifically, only activated Tregs were capable of successfully secreting BDNF after BDNF-mRNA electroporation. ABSTRACT Genetic engineering of regulatory T cells (Tregs) … Read more

BAFF blockade suppresses B cell MALT formation in H. felis-infected Nlrc5mø-KO mice. The upregulation of protein-coding BAFF transcripts in NLRC5-deficient macrophages suggests a novel NLRC5–BAFF signalling axis. ABSTRACT Helicobacter infection is a key cause of gastric B cell mucosa–associated lymphoid tissue (MALT) lymphoma. This study examined the role of B cell–activating factor (BAFF), a major … Read more

The number of exhausted T cells continuously increases in chronic sepsis. Both knockout and “knockdown” of TOX failed to alleviate sepsis-induced T-cell exhaustion. However, deletion of TOX impaired the effector function of T cells, suggesting that TOX is not involved in driving T-cell exhaustion in sepsis. ABSTRACT The immune system undergoes profound dysregulation in sepsis, … Read more