IgE-mediated activation of human monocytes results in SHIP-1 activation, IL-10 production, and inhibition of antiviral IP-10. SHIP-1 inhibition by 3AC enhanced IL-10 but suppressed antiviral IP-10. This indicates differential regulatory roles for SHIP-1, which could impact antiviral responses in allergic diseases. ABSTRACT IgE-mediated stimulation of monocytes regulates multiple cellular functions including cellular maturation, cytokine release, … Read more

We here provide detailed protocols for isolating single cell suspension from human nonlymphoid tissues, such as gingiva, lung, intestine, skin, and different tumor tissues as well as tumor-draining lymph nodes. Further, we provide gating strategies for flow cytometric analysis of human DC subpopulations in diverse nonlymphoid tissues. Abstract This article is part of the Dendritic … Read more

Chimeric antigen receptor (CAR) T-cells have proven effective in hematologic malignancies targeting a restricted set of tumor-associated antigens. In this work, we demonstrate that CAR-T cells can be redirected to alternative tumor antigens through a switch protein adapter with antitumor efficacy in preclinical models. ABSTRACT Chimeric antigen receptor-T cell (CAR-T) immunotherapy has shown remarkable results … Read more

Identifying activated T lymphocytes and discerning antigen-specific from bystander T cells is crucial for understanding immune responses. Dual AIM combinations significantly enhance the ability to distinguish antigen-specific from bystander-activated T cells compared with single AIMs. They also have a better positive predictive value and specificity with a modest reduction in sensitivity. ABSTRACT Identifying activated T … Read more

Tumor and immune cells interact through receptor–ligand interactions. B3GNT5 produces nsGSL on tumor cells which is regulated by SPPL3. When SPPL3 is absent, elevated nsGSL levels hinder receptor–ligand binding, leading to reduced immune cell activation. ABSTRACT The development of an effective antitumor response relies on the synergistic actions of various immune cells that recognize tumor … Read more

Novel computational framework tviblindi identifies branching B cell developmental trajectories in healthy (HD) and abnormal (RAG-1 and Wiskott–Aldrich syndrome primary immunodeficiency) bone marrow and peripheral blood samples using 30 parameter mass cytometry. Integrated vaevictis projection allows interpretation of trajectories to κ (grey arrow) and λ (black arrow) light chain expressing B cells, memory development branching … Read more

MiR-146a−/− mice are protected from lethal MCMV infection. This protection is dependent on miR-146a expression in the hematopoietic compartment and NK cells. miR-146a−/− mice have increased liver mature NK cells, STAT-1 phosphorylation, and Ly49H+ NK cell and T-cell expansion, which can contribute to protecting the host from lethal MCMV. Abstract Natural killer (NK) cells are … Read more

Diversification of transgenic TCRs by CRISPR/Cas9-mediated mutagenesis of Tcra and Tcrb chain genes in vivo converts monoclonal repertoires of known specificity into an oligoclonal pool of TCRs of altered antigen reactivities. This generally applicable approach helps to identify crucial amino acid residues in the CDR3 regions required for antigen recognition. ABSTRACT The T-cell receptor sequences … Read more

This review provides deeper insights into the localization of intra-tumoral γδ T lymphocytes in solid tumors. Additionally, a pro- or anti-tumoral influence of the tumor microenvironment and expression of checkpoint inhibitory molecules on γδ T cell functionality is described. To overcome the suppression of tumor-infiltrating γδ T cells, future immunotherapy approaches are summarized. ABSTRACT Human … Read more

Il1r2 deficiency in monocytes dysregulates their trafficking to inflamed tissues through increased production of the monocyte chemoattractant CCL2. ABSTRACT IL1-β plays a central role in inflammation but its biological action needs to be tightly controlled. Such negative regulation can be exerted by the decoy receptor IL1R2. However, IL1R2 biology in immune cells remains poorly characterized, … Read more