Severe COVID-19 is associated with hyperinflammation driven by anti-spike IgG immune complexes (ICs) contributing to macrophage hyperactivation, thrombosis, and tissue damage. This study shows that anti-spike IgG ICs induce rapid metabolic reprogramming, specifically glycolysis, fatty acid synthesis, and the pentose phosphate pathway, which is essential for the inflammatory response. Targeting these metabolic pathways may offer … Read more

Unannotated splicing and translation of non-canonical open reading frames generate a diverse repertoire of cryptic proteins. Most are rapidly degraded, rendering them possible MHC-I substrates. Yet, a subpopulation can adopt stable structures and impart advantageous functions. This dichotomy both expands the antigen landscape and provides a framework for protein evolution. ABSTRACT The widespread translation of … Read more

This is an update to the Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition), Chapter 3: 12C, by Cossarizza et al. Administration of anti-ARTC2 nanobody(S+16a) prevents cell death during tissue processing. We demonstrate that the phenotype of CD44midTreg is significantly impacted, whereas the eTreg phenotype remains stable following … Read more

Here, we show increased MHC class I levels on B cells, classical and intermediate monocytes, and a shift in CD8+ T cells toward a Tc1-skewed, proinflammatory phenotype in the peripheral blood mononuclear cells of people with type 2 diabetes mellitus. ABSTRACT Effective antigen presentation by major histocompatibility complex (MHC) molecules to T cells is crucial … Read more

HCV re-infection is common after spontaneous clearance of the initial infection. In the rare group of super-clearers who clear repeatedly, HCV-specific CD8+ T memory stem cells (TSCM) retain stemness, proliferation, and multipotency, sustaining immune protection. In contrast, clearer chronics lose these properties. TSCM self-renewal underpins durable immunity and should inform vaccine design. ABSTRACT Natural clearance … Read more

Selecting a sensitive test system is crucial to measure IFNα in serum samples. Simoa digital ELISA stands out for its high sensitivity and strong correlation with transcript-based IFN-induced gene (ISG) scores. In SLE, the ISG score is explained by circulating IFNα levels with negligible contribution from IFNβ or IFNγ. Created in BioRender.

Using mass cytometry, we map the heterogeneous activation of the STING pathway at single-cell resolution in cell lines and primary human immune cells and validate them with conventional techniques. ABSTRACT The stimulator of interferon genes (STING) pathway plays a pivotal role in innate immunity, acting as a key sensor of cytosolic DNA to initiate type-I … Read more

We optimised a protocol for mHA-specific Treg (mTreg) selection in an HLA-matched context while defining its phenotype, transcriptional state and function. mTreg were highly activated and exerted specific, HLA class II-, contact-dependent suppression. This protocol can be explored as a highly personalised antigen-specific Treg-based therapy in future clinical trials for cGVHD. ABSTRACT Graft-versus-host disease is … Read more

This review highlights the hierarchical transcriptional networks that govern macrophage specification and function. We describe how lineage-determining, identity-imprinting, and tissue-specific transcription factors coordinate macrophage differentiation and specialization across organs, emphasizing the transcriptional regulation underlying macrophage diversity in health and disease. ABSTRACT Tissue-resident and recruited macrophages are integral to organ development, homeostasis, immunity, and disease pathogenesis. … Read more

The Janus face of group 3 innate lymphoid cells (ILC3s). ILC3s are highly plastic and can adapt their immunoregulatory roles according to the local context, balancing tolerance and activation. Influenced by tissue-specific environmental cues, they can express MHC class II molecules either alone or jointly with costimulatory molecules, therefore presenting acquired antigens for controlling or … Read more