Establishing an untargeted lipidomics workflow for cellular analysis: insights into endothelial cell function in anaphylaxis

BackgroundCell metabolomics, including lipidomics, presents several challenges regarding analyzing limited cell populations and distinguishing cellular metabolites from background signals originated from a stimuli or after a treatment. To address this,…

PD-1 blockade does not enhance alloimmunization after allogeneic dendritic cell vaccination in cancer patients

BackgroundBlocking programmed cell death protein 1 (PD-1) has become a standard cancer immunotherapy, increasingly used in kidney, liver, or heart transplant recipients who develop skin cancer or hepatocellular carcinoma, despite the increased risk of …

Cloning, expression and characterisation of antigen-specific recombinant bat immunoglobulin from the black flying fox (Pteropus alecto)

Bats are natural reservoir of viruses that cause severe disease in livestock and humans. Recent high-profile spillover events have directed significant attention towards the relationship between zoonotic viruses and antiviral immunity inherent to bats….

Immune checkpoint inhibitor-induced bullous pemphigoid: a systematic review of clinical characteristics and outcomes based on case reports

ObjectiveImmune checkpoint inhibitor-induced bullous pemphigoid (ICI-BP) is a rare and complex cutaneous immune-related adverse event (cirAE) that often impacts the continuation of ICI therapy. Currently, there are no prospective clinical studies addre…

Prognostic value of baseline circulating tumor DNA levels in metastatic castration-resistant prostate cancer: a systematic review and meta-analysis

BackgroundMetastatic castration-resistant prostate cancer (mCRPC) remains a clinically aggressive and lethal disease. Circulating tumor DNA (ctDNA), as a minimally invasive biomarker, has shown prognostic utility in several solid tumors. However, its c…

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